Can I run more than one KASP genotyping assay per reaction plate?
It is possible to run a number of different KASP assays (i.e. for different SNPs / indels) on one reaction plate, as long as there are sufficient data points for each assay (minimum 22 plus 2 no template controls). For example, if performing KASP genotyping in 96-well plates, the same 22 DNA samples plus 2 NTCs could be represented up to four times on the plate. Each set of samples could be genotyped with a separate KASP Assay mix, thus enabling four different KASP assays to be run on the plate. The resultant fluorescent data should be analysed assay by assay – data points generated from different KASP assays should not be plotted on the same cluster plot.