What should I do if my qPCR instrument does not have an option to read HEX?
KASP™ chemistry is supplied with the FAM and HEX fluorophores for allelic discrimination. All of the ABI qPCR instruments (e.g. 7500, 7900, Viia7) have the ability to read VIC by default instead of HEX. VIC does, however, have the same excitation and emission spectra as HEX. When reading KASP genotyping reactions on one of these machines, ensure that you select VIC as a substitute for HEX.