What can I do if my DNA samples are not at a sufficient concentration for genotyping?
If you do not have sufficient genomic DNA for the number of SNPs that you wish to run, it is possible to perform Whole Genome Amplification (WGA). WGA is a PCR technique that is used to produce large quantities of DNA from a small amount of starting material. There are a number of methods for WGA, and LGC favours use of the primer extension pre-amplification (PEP) technique. PEP employs the use of randomly synthesised 15-mer oligonucleotides, referred to as polyN15, that bind at sites throughout the genome and act as primers to enable DNA replication. LGC uses our in-house enzyme, Klear Taq, and buffer system to perform WGA reactions.