How stable are the CAL Fluor® and BHQ® dyes when conjugated to peptides in solution?

CAL Fluor® dyes and BHQ® dyes are very stable when conjugated to peptides in solution and their pH stability is similar to that of the peptide in which they have been incorporated. Please note however that BHQ dyes are susceptible to reduction in certain solutions. DTT, TCIP, and other scavengers will reduce the azo bonds in the BHQ structure and should be avoided in peptide synthesis protocols that involve BHQ dyes.