FAQ

Is there any way of designating a specific region of my sequence for the RealTimeDesign™ software to design my primers, probe and assay?

RealTimeDesign™ software users may restrict assay design to include a specific sequence location, i.e. an intron splice site, by placing a tilde (~) character within the sequence.

For example: CAAAGGGTTGCAC~AAGATGGATGATCG