What does the 'Overall Rank' score in your RealTimeDesign™ software represent?
RealTimeDesign™ presents all candidate primers, probes, and assays in order of descending rank score. The rank score represents how closely the design matches the ideal values for each parameter setting. This aggregate value is principally used by the software to advance those candidate oligos that are most optimal, to ultimately present a single assay that is the most highly ranked.Note: Every assay proposed by RealTimeDesign is expected to function regardless of the rank score. In other words, there is no threshold or cut-off ranking below which all assays will under-perform.