What is the 'Failure Count Data' in your RealTimeDesign™ software?
The RealTimeDesign™ (RTD™) software uses a variety of parameter settings (e.g. amplicon length, melting temperature and GC percentage) in order to design optimal primers and probes. An oligo candidate will fail the design process if it falls outside the minimum or maximum values for each parameter limitation. The 'Failure Count Data' indicates how many candidates failed for each particular parameter. Editing individual parameters can overcome a failed assay design by increasing the number of oligo candidates, but may not function satisfactory in qPCR.