FAQ

I do not have genomic sequence for my particular organism. Can I use a closely related organism/species for my reference sequences?

Yes, however, the organism/species should be very closely related. It is the responsibility of the customer to ensure that the SNP locations in the polymorphism definition file match the SNPs in the species of interest. Note that differences between the reference genome provided and the genome sequenced may lead to lower than expected quality scores.

All Flex-Seq projects require:

  • a reference sequence (genomic or transcriptomic, or a reference scaffold). These can a provided as a link to a publically available genome, or submitted as a FASTA file.
  • a list of SNP locations to be interrogated, submitted as a VCF, Excel or BED file.