FAQ

My reference genome was assembled from fragment DNA sequencing, and is highly polymorphic (with possible unidentified SNPs in the flanking regions to my SNPs of interest). Would Flex-Seq work?

Generally, compared to other targeted genotyping methods, Flex-Seq is more tolerant of known or unknown SNPs in the probe binding region. However the quantity and location of the variants can have a negative impact on probe hybridization, and can lead to increased missing data.
When the flanking SNPs are unknown, and suspected to be high, we would recommend a trial study using the fragmented draft reference genome to assess the project feasibility. In terms of probe design, a mismatch that is located in the middle of the probe is typically acceptable.