I have limited amounts of DNA remaining for my project. What is the minimum DNA concentration I can submit without impacting library preparation too much?

We do not recommend lowering the DNA amount dramatically, as the library preparation has been validated to require 20 µL at 30 ng/µL. In case that the DNA is highly purified and > 10kb it is possible to submit concentrations of approximately 20 ng/µL but the volume should be no less than 20 µL. Please contact technical and applications support for further information.