I wish to screen F2 segregating populations in which each SNP can be both homozygous and heterozygous. Will SeqSNP provide enough depth of coverage to allow for this level of discrimination?
The standard SeqSNP coverage has predicted 20x per read to confidently call variants. This should be sufficient to discriminate genotypes in an F2 generation. Should a greater level of coverage be required, please contact technical and applications support, as this will be dependent on the specifics of your project.