Are Stellaris™ RNA FISH Probes comprised of DNA, RNA, or non-standard nucleotides?

Stellaris RNA FISH probes are comprised of DNA oligonucleotides, each singly-labeled with a fluorophore. Their simple structure and small size allow for sample permeabilization without protease treatment. As a result, the Stellaris method is less cumbersome than other FISH methods and more easily integrates with complementary detection techniques, such as immunofluorescence.