Can Stellaris RNA FISH probes detect DNA targets?
Stellaris RNA FISH probes hybridise to RNA. Genomic (double stranded) DNA remains inaccessible as no denaturation step is performed to unwind the DNA. Further, the Tm of the short, single-stranded probes is lower in DNA than in RNA and would therefore be competed away by the complementary strand. Simultaneous RNA and DNA detection using different probe types is not currently supported because the denaturation step needed to unwind DNA will remove the Stellaris RNA FISH probes.