What is the consequence (and cost) if I order a Stellaris® RNA FISH probe set with fewer than 48 oligos?
In general, the Stellaris RNA FISH method produces better results when using probe counts over 25. By default, the software proposes up to 48 probes when the transcript is sufficiently long. If ordering fewer than 48 probes, the quantity of each oligonucleotide in a Stellaris RNA FISH Probe set will vary to maintain a final blended amount of 5 nmol. The cost of a Stellaris RNA FISH Probe set remains the same regardless of the number of distinct sequences in the probe set.