The 3-TAM modification incorporates a fluorescent tetramethylrhodamine (TAMRA) at the 3' terminus of an oligonucleotide. TAMRA modified oligonucleotides can be used in a wide array of applications, including dual-labeled fluorogenic probes for real-time PCR. TAMRA is a rhodamine dye and this product is prepared using the 6-carboxy isomer. The dye is conjugated via a polyethylene glycol (PEG) linker to a modified cytidine which may participate in hybridization. The 3-TAM modification also introduces a 3' phosphate which prevents extension of the oligonucleotide by DNA polymerase. TAMRA fluoresces in yellow-orange region of the visible spectrum and can be effectively quenched by BHQ®-2 dye.
Properties:
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Chemical Name:
TAMRA
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Formula:
C45H55N6O14P
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Molecular Weight:
934.92
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Absorption Maximum (Lambda Max):
557
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Extinction Coefficient at Lambda max:
90000
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Extinction Coefficient at 260 nm:
39030
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Fluorescence Maximum:
583
Product usage:
-
The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
998.91
