Nucleic acid isolation kits for molecular diagnostics
Our suite of DNA and RNA extraction and purification reagents yield high-quality nucleic acids, without burdening your team with lengthy protocols and complex workflows. Our experts are readily available to guide the right selection for your needs or we customise a novel solution in our ISO-certified facilities.
Choose from a variety of kits depending on whether you require:
A fast and simple protocol that saves time and money
Crude extractions suited for high-throughput genotyping by PCR
Highly purified nucleic acids for more sensitive downstream applications (like NGS)
A fully automatable chemistry to increase throughput
Compatibility with a variety of sample types, including difficult samples such as FFPE
High yields of DNA and various RNAs
Non-hazardous, safe kit reagents
QuickExtract™ DNA Extraction Solution
This DNA extraction solution can rapidly and efficiently extract PCR-ready genomic DNA from many samples using a simple, one-tube protocol and non-toxic reagents. Our partners have used this solution on samples that include hair follicles and buccal cells.
QuickExtract is right for you if:
- You need a fast and easy protocol supporting various sample types.
- Crude nucleic acid extraction is sufficient for your application like PCR (typically not for NGS or other sensitive downstream applications).
- You do not require a fully automated solution, but still need high throughput.
Example applications: PCR analyses in applications such as genomic or viral DNA screening in human samples, T7E1 CRISPR mutation detection assays.
QuickExtract™ RNA Extraction Solution
The QuickExtract RNA Extraction Solution is a fast, simple way of preparing RNA for RT-PCR (both endpoint and real-time). This kit uses a single-tube system with non-toxic reagents that only requires vortex mixing to lyse the cells and prepare the RNA for cDNA synthesis. The kit works with cells from human samples. It is however not suitable for tissue samples. An optional DNase I treatment may improve certain downstream applications.
Example applications: cDNA synthesis for PCR and NGS applications, end-point and real-time RT-PCR.
mag™ and sbeadex™ Kits
The mag and the sbeadex kits (e.g. sbeadex blood, sbeadex forensic, mag mini/maxi and mag nanogram) use magnetic beads to purify DNA from a variety of blood sample types such as EDTA heparin, citrate and buffy coats as well as swab matrices, tissue and forensic samples. They support automated DNA extractions using magnetic particle handlers, as well as liquid handlers. While our mag kits are based on proven, conventional magnetic beads, sbeadex kits use an innovative next-generation technology of double-coated magnetic beads that yield improved DNA quality and omit enzyme inhibiting organic solvents in the final eluate.
mag and sbeadex kits are right for you if:
- Highly purified nucleic acids are required for a variety of downstream applications like PCR or NGS.
- A fully automatable solution is needed to support high-throughput workflows.
Example applications: Enzymatic fragmentation in NGS applications, PCR analyses, Sanger sequencing.
sbeadex™ Pathogen Nucleic Acid Purification Kit
This kit delivers excellent yields of highly purified DNA and RNA from pathogens collected in swab or sputum samples. It is excellent for pathogen-testing protocols including SARS-CoV-2, influenza and easy-to-lyse bacterial pathogens.
Example applications: PCR/qPCR, RT-PCR/qRT-PCR, NGS.
MasterPure™ Complete DNA and RNA Purification Kit
MasterPure purification kits use non-hazardous reagents to purify total nucleic acid (TNA), high molecular weight genomic DNA, as well as total cellular RNA (large and small RNAs) from many different sample types, including FFPE samples, blood, bacteria and viruses.
MasterPure Kits are right for you if:
- You require high yield, high purity nucleic acids
- You need compatibility for a wide range of sale types
- You do not require a fully automated setup where throughput is not a priority
Example applications: qPCR, library preparation for sequencing of genomic DNA and RNA, DNA methylation studies, genomic DNA and cDNA cloning, microarray analyses (CGH, gene expression profiling, etc.)
Kleargene™ Nucleic Acid Purification Kits
The silica-based Kleargene chemistry has been developed for manual or semi-automated high-throughput nucleic acid purification from a broad range of sample materials, including blood, tissues and saliva. It has been optimised for high-yield isolation of highly purified DNA suited for many downstream applications.
Kleargene kits are right for you if:
- You require highly pure DNA in high yields
- Your throughput needs don’t exceed 96 or 384 multiwell formats
- You have to process large volume samples (e.g. blood samples up to 10 mL)
Example applications: PCR, SNP genotyping, cloning, sequencing.
Enzymes and master mixes for PCR-based molecular diagnostics
RapiDxFire™ Thermostable Reverse Transcriptase
This highly thermostable, proprietary reverse transcriptase was first identified in a virus isolated from a hot spring. It performs fast and efficient first-strand cDNA synthesis using gene-specific primers. For MDx developers, its rapid reaction time (5 minutes or less) can support a streamlined RT-qPCR workflow and faster time to results.
RapiDxFire qPCR 5X Master Mix GF
A high-performing lyo-compatible master mix for sensitive pathogen detection (down to ~10 genomic DNA copies). Formulated with diagnostic kit developers in mind, this master mix can be combined with gene-specific primers and hydrolysis probes for immediate use in high-throughput laboratory developed test (LDT) workflows or further processed for lyophilisation and use in point-of-care (POC) devices. With a wide dynamic range, this product is also a good fit for multiplexed tests.
RapiDxFire Hot Start Taq DNA Polymerase
This polymerase activates in as little as 15 seconds, delivering sensitive detection of low abundance target DNA (down to ~10 genomic DNA copies). Ideal for standard end-point PCR and qPCR applications, including biomarker discovery and monitoring, gene expression analyses, SNP genotyping and other diagnostic tests.
Enzymes and reagents for NGS-based molecular diagnostics
High-quality, high-yield nucleic acids and efficient library prep are central to the success of next-generation sequencing (NGS). Our dedicated teams will work with you to provide specialty sample preparation and library preparation components for your NGS-based workflows.