The IntelliQube® features a fully integrated automated PCR and qPCR setup, amplification and data analysis.

Liquid handling

The IntelliQube allows multi-functional dispensing for high speed fluid handling in 384- or 768-well Array Tape®. An interchangeable CyBi FeliX 96- or 384-channel pipetting head precisely dispenses DNA samples into Array Tape.

IntelliQube has an integrated pipette wash system reducing tip waste and associated consumable costs while mitigating the risk of cross contamination.

Amplification 

The IntelliQube integrated amplification is supported by a Peltier thermal cycling block.

  • Designed specifically for 384- and 768-well Array Tape, (no block change is required)
  • Excellent ramp rates
  • Temperature uniformity and accuracy enable broad compatibility with amplification methodologies.

Detection

  • An advanced optical system optimised for integrated fluorescence detection
  • Filtered LEDs are optimised for a variety of commonly used fluorophores (range of 480-620 nm)
  • A high-resolution CCD camera supports detection of fluorescence intensity within 5 detection channels for dyes emitting within the range of 510-705 nm
  • Multiplex data capture for all 5 optical channels can occur in as little as 10 seconds.

Software and data analysis

The Intellics® Software Suite provides:

  • Centralised data management
  • Instrument monitoring
  • Intelligent run optimisation
  • Protocol generation and streamlined data analysis. 

Intellics is pre-loaded on an embedded Windows® operating system, accessible to users through a unified web-based interface.

Intellics Array Builder allows users to rapidly generate liquid handling and thermal cycling protocols, and IntelliScore® for post-PCR data analysis and interpretation.

IntelliScore supports absolute quantification (standard curve), relative quantification (relative standard curve and ΔΔCq), genotyping, and presence / absence. CSV data export for LIMS systems is possible.

A touch screen interface facilitates instrument control and also supports real-time data monitoring.

For Research Use Only. Not for use in diagnostic procedures

Performance chart Sample Dispensing CyBi® FeLiX pipette head Assay Dispensing Dispense Jet
Mechanism Air displacement Single jet solenoid valve
Dispensing configuration 96- or 384-channel 4-channel
Dispense volume 0.8 µL
Precision  ≤5% 
Recommended input Nucleic acid templates 2X Primer+Probe+Mastermix
Source plate positions 10 (ambient temperature) 3 (temperature controlled)
Supported source plates ANSI/SBS compliant 96/384-well formats ≤15 mm in height ANSI/SBS compliant 96-well formats ≤ 30 mm in height
Total dispensing time Approximately 5 to 6 minutes per array

Temperature uniformity

Specification Peltier block type
Block configuration 384/768-well Array Tape
Temperature range 20 - 100°C
Temperature accuracy ±0.25°C
Temperature uniformity ±0.5°C at 60°C
Heating ramp rate 3.0°C/sec
Cooling rate 3.0°C/sec

Detection

LEDs
(460-645 nm)
Dye channel 1 2 3 4 5
Excitation filter (nm) 480 530 548 580 620
Emission Filter (nm) 510 565 580 625 705
Dyes with expected compatability FAM™
JOE™
VIC®/HEX
Cal Fluor®
Orange 560
NED™
TAMRA
Cal Fluor® Red 590
ROX™
Cal Fluor® Red 610
Texas Red®
Cy5®
Quasar® 670