Patented, PCR based KASP genotyping technology has been a critical tool for plant and livestock breeders for the last fifteen years, and the ability to screen KASP markers has underpinned breeding programs at industry leading AgBio companies. In the human genotyping world, KASP markers have been used in large cohort research studies to understand human health and disease.

As the AgBio industry moves towards looking at larger numbers of markers in different plant and animal species, and human health research learns more about the genetics underpinning common diseases, scientists at LGC continue to develop improved genotyping tools compatible with KASP technology.

KASP-TF Master Mixes are formulated without Triton™ X-100. We use KASP-TF Master Mix in our own, largest genotyping lab in Europe, so we can troubleshoot any KASP issues in house with our experts using the same instruments as our customers, and  bring assays in house at times of peak demand or customer laboratory failures.

Key features of KASP-TF Master Mix supply and production

  • Each batch of KASP-TF Master Mix is functionally tested in high throughput genotyping
  • Patent-protected KASP technology has undergone continuous development to incorporate customer performance feedback to optimize speed and performance
  • Our stable supply chain includes disaster recovery processes, allowing us to reliably supply large volumes of KASP-TF Master Mix to large, commercial customers
  • Global teams of sales, customer service and technical support specialists, provide regional support for your product
  • Our complete workflow solutions include instruments, software, and upstream and downstream technologies developed with KASP genotyping customers in mind, with fully validated protocols
  • Order KASP-by-Design (KBD) assays to perform your own validation or KASP-on-Demand (KOD) for fully validated assays delivered by our in-house laboratory

 

Ordering

KASP-TF Master Mix is available in Low, Standard and High ROX as different qPCR instruments have differing requirements for ROX. Please refer to the KASP genotyping protocols for your PCR instrument to select the correct KASP-TF Master Mix for your instrument. 

KASP-TF Master mix can be ordered independently or in conjunction with your KASP assay. Review our KASP Reagents Guidance Notes for step-by-step guide to ordering KASP reagents and deliver information, or request a quote or enquire about pricing, stating the instrument that you intend to perform your genotyping reactions on. The technical support team will ensure you receive the optimal version of KASP-TF Master Mix for your instrument. If you have questions, please contact our technical support team.

Don't see your catalog number? Use this reference table

 

Catalogue number

Description

ROX Level

Size

Format

KBS-1050-101

KASP-TF V4.0 2X Master Mix

Standard

2.5 mL

96/384

KBS-1050-102

KASP-TF V4.0 2X Master Mix

Standard

25 mL

96/384

KBS-1050-103

KASP-TF V4.0 2X Master Mix

Standard

250 mL (10 x 25 mL)

96/384

KBS-1050-104

KASP-TF V4.0 2X Master Mix

Standard

250 mL

96/384

KBS-1050-111

KASP-TF V4.0 2X Master Mix

Standard

2.5 mL

1536

KBS-1050-112

KASP-TF V4.0 2X Master Mix

Standard

25 mL

1536

KBS-1050-113

KASP-TF V4.0 2X Master Mix

Standard

250 mL (10 x 25 mL)

1536

KBS-1050-114

KASP-TF V4.0 2X Master Mix

Standard

250 mL (5 x 50 mL)

1536

KBS-1050-115

KASP-TF V4.0 2X Master Mix

Standard

250 mL

1536

KBS-1050-116

KASP-TF V4.0 2X Master Mix

Standard

25 mL (2 x 125 mL)

1536

KBS-1050-121

KASP-TF V4.0 2X Master Mix

Low

2.5 mL

96/384

KBS-1050-122

KASP-TF V4.0 2X Master Mix

Low

25 mL

96/384

KBS-1050-123

KASP-TF V4.0 2X Master Mix

Low

250 mL (10 x 25 mL)

96/384

KBS-1050-124

KASP-TF V4.0 2X Master Mix

Low

250 mL

96/384

KBS-1050-131

KASP-TF V4.0 2X Master Mix

High

2.5 mL

96/384

KBS-1050-132

KASP-TF V4.0 2X Master Mix

High

25 mL

96/384

KBS-1050-133

KASP-TF V4.0 2X Master Mix

High

250 mL (10 x 25 mL)

96/384

KBS-1050-201

KASP-TF V5.0 2X Master Mix

Standard

2.5 mL

Array Tape

KBS-1050-202

KASP-TF V5.0 2X Master Mix

Standard

25 mL

Array Tape

KBS-1050-203

KASP-TF V5.0 2X Master Mix

Standard

250 mL (10 x 25 mL)

Array Tape

KBS-1050-204

KASP-TF V5.0 2X Master Mix

Standard

250 mL

Array Tape

 

For research use only. Not for use in diagnostic procedures.

KASP-TF Master Mix contains the universal FRET cassettes, ROX™ passive reference dye, Taq polymerase, free nucleotides and MgCl2 in an optimised buffer solution. The KASP-TF Master Mix is universal and can be used in conjunction with all KASP Assays. 

 

Title Description  
KASP manual Full user guide for running KASP in your own laboratory Download
KASP quick start guide Outline protocol for set up of KASP genotyping reactions Download 
KASP recycling quick guide KASP genotyping - guide to further cycling of genotyping reactions Download
How does KASP work? Overview of how KASP genotyping chemistry works and how data is scored at LGC Download
How to submit sequences A guide to how sequence information should be submitted to LGC for KASP assay design Download
KASP assay design for highly similar sequences An explanation of how KASP assays can be designed with an anchor, to differentiate between two highly similar sequences Download
How much DNA should I send for my genotyping project? This document outlines the amount of DNA required for a genotyping project run in our service laboratories Download
Reference list Partial list of species that have been genotyped using KASP Download
Trial kit manual User guide for running the KASP genotyping trial kit Download
Trial kit quick start guide Outline protocol for running the KASP genotyping trial kit Download
Validation kit manual User guide for running the KASP Genotyping validation kit (only sent to customers that are using instruments that we are not familiar with). Download
Running KASP on the ABI 7500 User manual for setting up and running KASP on the ABI 7500 qPCR instrument Download
Running KASP on the ABI 7900 User manual for setting up and running KASP on the ABI 7900 qPCR instrument Download
Running KASP on the ABI StepOne and StepOne Plus User manual for setting up and running KASP on the ABI StepOne and StepOne Plus qPCR instrument Download
Running KASP on the ABI Viia7 User manual for setting up and running KASP on the ABI ABI Viia7 qPCR instrument Download
Running KASP on the BIO-RAD CFX User manual for setting up and running KASP on the BIO-RAD CFX qPCR instrument Download
Running KASP on the Roche LC480 User manual for setting up and running KASP on the Roche LC480 qPCR instrument Download
Running KASP on the illumina EcoRT User manual for setting up and running KASP on the illumina EcoRT qPCR instrument Download
Running KASP on the Agilent Mx3000 / Mx3005P User manual for setting up and running KASP on the Agilent Mx3000 / Mx3005P instrument Download
Running KASP on Roche LC96 Factsheet gives detailed explanation of the issues
running KASP genotyping on an LC96 instrument
Download
KASP thermal cycling conditions A document containing details of all the commonly used thermal cycling protocols for KASP genotyping. Download
KASP thermal cycling 61-55ºC touchdown protocol The standard KASP thermal cycling conditions.   Download
KASP thermal cycling 68-62ºC touchdown protocol An alternative KASP thermal cycling protocol, typically used for assays with high %GC. Download
KASP thermal cycling 2-step 57ºC protocol An alternative KASP thermal cycling protocol, typically used for assays with low %GC. Download
KASP thermal cycling 65-57ºC touchdown protocol The standard KASP thermal cycling conditions for the Fluidigm platform. Download
KASP thermal cycling recycling protocol The thermal cycling conditions for further cycling (‘recycling’) of KASP genotyping reactions. Download
Running KASP on Thermo Scientific Piko Real User manual for setting up and running KASP on the Thermo Scientific PikoReal instrument Download

Find your recommended KASP-TF Master Mix in the table below. You can also refer to the KASP genotyping protocols for your PCR instrument to select the correct KASP-TF Master Mix for your instrument.

Discontinued KASP Master Mixes New KASP-TF Master Mixes
Catalog Number Description Catalog number Description
KBS-1016-001 KASP V4.0 2X Master Mix 96/384, Standard ROX, 500 x 10 μL Reactions - 2.5 mL KBS-1050-101 KASP-TF V4.0 2X Master Mix 96/384, Standard ROX (2.5 mL)
KBS-1016-001-US KASP V4.0 2X Master Mix 96/384, Standard ROX, 500 x 10 μL Reactions - 2.5 mL USA/AU
KBS-1016-002 KASP V4.0 2X Master Mix 96/384, Standard ROX, 5 000 x 10 μL Reactions - 25 mL KBS-1050-102 KASP-TF V4.0 2X Master Mix 96/384, Standard ROX (25 mL)
KBS-1016-002-US KASP V4.0 2X Master Mix 96/384, Standard ROX, 5 000 x 10 μL Reactions - 25 mL USA/AU
KBS-1016-003 KASP V4.0 2X Master Mix 96/384, Standard ROX, 50 000 x 10 μL Reactions - 250 mL KBS-1050-103 KASP-TF V4.0 2X Master Mix 96/384, Standard ROX (250 mL) (10 x 25 mL)
KBS-1016-003-US KASP V4.0 2X Master Mix 96/384, Standard ROX, 50 000 x 10 μL Reactions - 250 mL - 10 x 25 mL USA/AU
KBS-1016-003B-US KASP V4.0 2X Master Mix 96/384, Standard ROX, 50 000 x 10 μL Reactions - 250 mL - 1 x 250 mL USA/AU KBS-1050-104 KASP-TF V4.0 2X Master Mix 96/384, Standard ROX (250 mL)
KBS-1016-010 KASP V4.0 2X Master Mix 1536, Standard ROX, 5 000 x 1 μL Reactions - 2.5 mL KBS-1050-111 KASP-TF V4.0 2X Master Mix 1536, Standard ROX (2.5 mL)
KBS-1016-010-US KASP V4.0 2X Master Mix 1536, Standard ROX, 5 000 x 1 μL Reactions - 2.5 mL USA/AU
KBS-1016-011 KASP V4.0 2X Master Mix 1536, Standard ROX, 50 000 x 1 μL Reactions - 25 mL KBS-1050-112 KASP-TF V4.0 2X Master Mix 1536, Standard ROX (25 mL)
KBS-1016-011-US KASP V4.0 2X Master Mix 1536, Standard ROX, 50 000 x 1 μL Reactions - 25 mL USA/AU
KBS-1016-012 KASP V4.0 2X Master Mix 1536, Standard ROX, 500 000 x 1 μL Reactions - 250 mL - 10 x 25 mL KBS-1050-113 KASP-TF V4.0 2X Master Mix 1536, Standard ROX (250 mL) (10 x 25 mL)
KBS-1016-012-US KASP V4.0 2X Master Mix 1536, Standard ROX, 50 000 x 1 μL Reactions - 250 mL - 10 x 25 mL USA/AU
KBS-1016-012B KASP V4.0 2X Master Mix 1536, Standard ROX, 500 000 x 1 μL Reactions - 250 mL - 1 x 250 mL KBS-1050-115 KASP-TF V4.0 2X Master Mix 1536, Standard ROX (250 mL)
KBS-1016-012B-US KASP V4.0 2X Master Mix 1536, Standard ROX, 500 000 x 1 μL Reactions - 250 mL - 1 x 250 mL USA/AU
KBS-1016-016 KASP V4.0 2X Master Mix 96/384, Low ROX, 500 x 10 μL Reactions - 2.5 mL KBS-1050-121 KASP-TF V4.0 2X Master Mix 96/384, Low ROX (2.5 mL)
KBS-1016-016-US KASP V4.0 2X Master Mix 96/384, Low ROX, 500 x 10 μL Reactions - 2.5 mL USA/AU
KBS-1016-017 KASP V4.0 2X Master Mix 96/384, Low ROX, 5 000 x 10 μL Reactions - 25 mL KBS-1050-122 KASP-TF V4.0 2X Master Mix 96/384, Low ROX (25 mL)
KBS-1016-017-US KASP V4.0 2X Master Mix 96/384, Low ROX, 5 000 x 10 μL Reactions - 25 mL USA/AU
KBS-1016-018 KASP V4.0 2X Master Mix 96/384, Low ROX, 50 000 x 10 μL Reactions - 250 mL KBS-1050-123 KASP-TF V4.0 2X Master Mix 96/384, Low ROX (250 mL) (10 x 25 mL)
KBS-1016-018-US KASP V4.0 2X Master Mix 96/384, Low ROX, 50 000 x 10 μL Reactions - 250 mL - 10 x 25 mL USA/AU
KBS-1016-018B-US KASP V4.0 2X Master Mix 96/384, Low ROX, 50 000 x 10 μL Reactions - 250 mL - 1 x 250 mL USA/AU KBS-1050-124 KASP-TF V4.0 2X Master Mix 96/384, Low ROX (250 mL)
KBS-1016-021 KASP V4.0 2X Master Mix 96/384, High ROX, 500 x 10 μL Reactions - 2.5 mL KBS-1050-131 KASP-TF V4.0 2X Master Mix 96/384, High ROX (2.5 mL)
KBS-1016-021-US KASP V4.0 2X Master Mix 96/384, High ROX, 500 x 10 μL Reactions - 2.5 mL USA/AU
KBS-1016-022 KASP V4.0 2X Master Mix 96/384, High ROX, 5 000 x 10 μL Reactions - 25 mL KBS-1050-132 KASP-TF V4.0 2X Master Mix 96/384, High ROX (25 mL)
KBS-1016-022-US KASP V4.0 2X Master Mix 96/384, High ROX, 5 000 x 10 μL Reactions - 25 mL USA/AU
KBS-1016-023 KASP V4.0 2X Master Mix 96/384, High ROX, 50 000 x 10 μL Reactions - 250 mL KBS-1050-133 KASP-TF V4.0 2X Master Mix 96/384, High ROX (250 mL) (10 x 25 mL)
KBS-1016-023-US KASP V4.0 2X Master Mix 96/384, High ROX, 50 000 x 10 μL Reactions - 250 mL USA/AU
KBS-1030-001 KASP Array Tape 2X Master Mix, Standard ROX, - 2.5 mL KBS-1050-201 KASP-TF V5.0 2X Master Mix, Standard ROX (2.5 mL)
KBS-1030-002 KASP Array Tape 2X Master Mix, Standard ROX, - 25 mL KBS-1050-202 KASP-TF V5.0 2X Master Mix, Standard ROX (25 mL)
KBS-1030-004 KASP Array Tape 2X Master Mix, Standard ROX, - 250 mL (10 x 25 mL) KBS-1050-203 KASP-TF V5.0 2X Master Mix, Standard ROX (250 mL) (10 x 25 mL)
KBS-1030-003 KASP Array Tape 2X Master Mix, Standard ROX, - 250 mL KBS-1050-204 KASP-TF V5.0 2X Master Mix, Standard ROX (250 mL)