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High-throughput, high-quality DNA and RNA purification for detection of multiple pathogens

Pathogen testing protocols (e.g. for COVID-19, influenza or easy-to-lyse bacterial pathogens) require fast, reliable, high-yield, high-quality and easily automatable sample preparation methods.

Our sbeadex Pathogen Nucleic Acid Purification Kits with their optimised chemistry meet these requirements and deliver excellent yields of highly purified nucleic acids from various sample material that can be used in many downstream applications such as PCR/qPCR, RT-PCR/qRT-PCR, and NGS. The purification procedure can be fully automated and is compatible with many robotic platforms.

The sbeadex Pathogen Nucleic Acid Purification Kit is a simple renaming of our proven sbeadex Viral RNA Purification Kit to reflect its continuously expanding usability to purify good yields of high-quality DNA and RNA from a variety of viral, bacterial and yeast pathogens.

Please see Table 1 for a list of viral, bacterial and yeast pathogens and compatible matrices that have been tested so far.

For Research Use Only. Not for use in diagnostic procedures.

Key benefits

  • Qualified protocol for viral and bacterial nucleic acid purification
  • Compatible with most popular robotic platforms, including the Kingfisher and our high-throughput oKtopure instrument
  • Fast – both short (25 min) and standard (45 min) protocols available
  • High-throughput batch sizes (up to 10,000 purifications)
  • No organic extraction, alcohol precipitation or centrifugation steps
  • Water-based wash buffers reduce solvent carryover, resulting in absence of PCR inhibitors in final eluate
  • High nucleic acid quality for all downstream applications including PCR/qPCR and NGS
  • Ready-to-use kits with high stability (customised and bulk size kits are also available)
Pathogen External Control (Artificial Matrix)
Viral Targets  
SARS-CoV-2 VTM1
Influenza A H3N2 Swab2
Influenza B Swab2
Novel Influenza A H1N1 Swab2
Respiratory Syncytial Virus (subtype A) Swab2
Respiratory Syncytial Virus (subtype B) Swab2
Adenovirus 4 Swab2
Zika Virus Plasma3
Cytomegalovirus Plasma4
Human Papillomavirus-16 Methanol-preserved cultured human cells containing full-length HPV episomal DNA5
Bacterial Targets  
Bordetella pertussis Swab6
Chlamydophila pneumoniae Swab6
Chlamydophila psittaci Swab6
Coxiella burnetii Swab6
Legionella pneumophila Swab6
Mycoplasma pneumoniae Swab6
Mycobacterium tuberculosis Sputum7
Campylobacter jejuni Stool8
Salmonella enteritidis Stool8
Yersinia enterocolitica Stool8
Clostridium difficile Stool8
Chlamydia trachomatis Urine9
Neisseria gonorrhoeae Urine9
Staphylococcus aureus External Run Control10
Other Targets
Candida albicans Positive Extraction Control11
Compatible Matrices Tested Swabs (UTM®/VTM)
Sputum
Saliva
Blood
Plasma
Serum
Urine
Stool
Cerebrospinal Fluid

Table 1: Pathogens that have already been tested using the sbeadex Pathogen Nucleic Acid Purification Kit.

Standards used for testing

  1. AccuPlex™ SARS-CoV-2 Verification Panel - Full Genome, 0505-0168 (LGC SeraCare)
  2. AMPLIRUN® TOTAL RESPIRATORY VIRAL PANEL CONTROL (SWAB), MBTC020 (Vircell S.L.)
  3. AMPLIRUN TOTAL ZIKV/DENV/CHIKV CONTROL (PLASMA), MBTC023 (Vircell S.L.)
  4. AMPLIRUN TOTAL CMV CONTROL (PLASMA), MBTC018-R (Vircell S.L.)
  5. AccuTrak™ HPV Genotype Qualification Panel, QSH701 (2400-0161), (LGC SeraCare)
  6. AMPLIRUN TOTAL ATYPICAL BACTERIAL PNEUMONIA CONTROL (SWAB) MBTC022-R (Vircell S.L.)
  7. AMPLIRUN TOTAL MTB CONTROL (SPUTUM) MBTC013 (Vircell S.L.)
  8. AMPLIRUN TOTAL GASTROINTESTINAL BACTERIAL PANEL CONTROL (STOOL), MBTC021 (Vircell S.L.)
  9. AMPLIRUN TOTAL CT/NG CONTROL (URINE), MBTC003 (Vircell S.L.)
  10. NATtrol™ S. aureus (MRSA) External Run Control, NATSAU(MRSA)-ERCM (Zeptometrix Corporation)
  11. NATtrol Candida/TV Positive Control, NATCTVPOS-BD (Zeptometrix Corporation)

Note: Other sample matrices may also be used with this kit. Further testing on other pathogens and matrices is in progress and this table will be updated accordingly.

Product name Item name Number of purifications per kit  
sbeadex Pathogen Nucleic Acid Purification Kit NAP40-024-01 96 Order now
NAP40-024-02 960
NAP40-024-03 5000 
NAP40-024-04 10000 
sbeadex Pathogen Nucleic Acid Purification Kit, no Proteinase K NAP40-026-01 96 Order now
NAP40-026-02 960
NAP40-026-03 5000 
NAP40-026-04 10000

Note: We also offer a no dangerous goods option and a no dangerous goods with no proteinase K option for Asia Pacific.

We provide customised solutions as well as very economical bulk sizes. Please contact us to support you establishing your individual setups or offering you individually tailored kits.

Request your test kit (96 preparations) now for 50% off!

Product info

sbeadex Pathogen Nucleic Acid Purification Kits are magnetic-bead based kits that are validated for research use only. The sbeadex pathogen nucleic acid purification portfolio offers products for all scales of nucleic acid preparation from buccal swab and sputum samples, eluting into 100 µL buffer.

DNA/RNA preparation protocol

sbeadex beads allow efficient and high-quality purification of small and large DNA and RNA fragments within 45 minutes without centrifugation steps. The beads are double coated, resulting in a unique dual binding protocol that allows elution of ultrapure nucleic acids into water, reducing solvent carryover:

  sbeadex Pathogen Nucleic Acid Purification Kit Competitor A
  N Average Cq StDev Average Cq StDev
Clean up sample 12 30.35 0.14 33.80 0.15
Direct spike-in sample 3 31.00 0.44 33.80 0.12

Figure 1:  Demonstration of no loss of RNA during the sbeadex Pathogen Nucleic Acid Purification Kit protocol.  500 copies of a 2019-COVID artificial RNA reference material were added to a sample (nasopharyngeal swab) and the sample processed with the sbeadex Pathogen Nucleic Acid Purification Kit. SARS-CoV-2 was detected in this preparation and a directly spiked positive control sample via a RT-qPCR at two separate Biosearch Technologies sites (reaction components described below). The concordant Cq values from both samples demonstrate no loss of sample due to the extraction step.

Recommended products

RT-qPCR reagents* including master mix and enzyme for SARS-CoV-2 assay development are also available.

Item name  
RapiDxFire 1-step RT-qPCR System View product
QuickExtract RNA Kit View product
QuickExtract DNA Kit View product

Probes and primers that target SARS-CoV-2

Item name  
Influenza SARS-CoV-2 Multiplex ValuPanel Reagents View product
Charité 2019-nCoV BHQ ValuPanel Reagents* View product
Charité 2019-nCoV BBQ-650 ValuPanel Reagents* View product
Yale 2019-nCoV SalivaDirect ValuPanel Reagents* View product

For a complete SARS-CoV-2 Quality Solutions, consider the AccuPlex SARS-CoV-2 Verification Panel coupled with the AccuPlex SARS-CoV-2 Reference Material Kit from LGC SeraCare Life Sciences. The target sequence of interest is packaged into a stable non-infectious, and replication-deficient alphavirus particle and the products contain positive materials directed against the published CDC and WHO consensus sequences.