Run one of our free-of-charge trial kits on your qPCR instrument before you running your own DNA samples and KASP assays.learn more
KASP genotyping chemistry
On June 01, 2020 the part numbers for KASP™ by Design (KBD) and KASP on Demand (KOD) assays will be updated.
KASP on Demand includes design, full validation and optimisation of your assay by our scientists prior to delivery. For human research applications it is advisable to use KOD.learn more
KASP by Design (KBD) consists of three KASP primers that are specific to the SNP or InDel of interest.learn more
Order our unique KASP™ formulation for use with the specialist optics and reduced sample volumes used in the Fluidigm genotyping system. Validation service is available.learn more
KASP™ is our proprietary genotyping technology.
The KASP genotyping reaction comprises three components:
- Sample DNA with the sequence of interest
- KASP Assay mix (containing the target specific primers)
- KASP Master mix.
The KASP Assay mix is specific to the SNP or InDel to be targeted and consists of two competitive, allele-specific forward primers and one common reverse primer. Each forward primer incorporates an additional tail sequence that corresponds with one of two universal FRET (fluorescent resonance energy transfer) cassettes present in the KASP Master mix. All custom KASP assays are available with the additional assay validation service.
KASP Master mix contains the two universal FRET cassettes (FAM and HEX), ROX™ passive reference dye, Taq polymerase, free nucleotides and MgCl2 in an optimised buffer solution.