KASP by Design (KBD) consists of three KASP primers that are specific to the SNP or InDel of interest. Assay design (primer selection) is carried out using our proprietary Kraken™ software system.

Please note that using in-silico validated primers cannot guarantee a working assay. The success rate for custom KASP assay design depends on a number of factors including DNA quality, genome complexity, homology and genome size. Any custom KASP assay can be provided with our additional assay validation service to ensure a working assay.

Ordering

  1. Please request a quotation or enquire about pricing.
  2. We will email you with the quotation for your assays and include a sequence submission form. For new customers we will also send a new account application form.
  3. Review your quotation and if you're happy with it, submit a purchase order to the address provided.
  4. Next, complete and return the sequence submission form with your sequence/s of interest and polymorphism/s marked to tell us which SNPs / InDels you are interested in.
  5. Assay design (primer selection) will then be carried out using our proprietary Kraken™ software suite.
  6. All three target-specific primers for an assay are combined into a single 2D barcoded tube.
  7. Each custom KBD Assay mix is shipped in a 2D barcoded tube, with an additional printed label.

Delivery

Aliquots of KBD Assay mix are provided in 2D barcoded tubes in sufficient quantities to enable:

  • 2,500 reactions at 10 µL total reaction volumes (96-well PCR plates)
  • 5,000 reactions at 5 µL total reaction volumes (384-well PCR plates).

Standard delivery times for KBD Assay mix is 2-3 weeks. Please note that you will require KASP Master mix in order to run your genotyping reactions. Please refer to the KASP Master mix – PCR instrument guide to select the correct KASP Master mix for your instrument. Should you have any questions at all, please contact us our customer services team or our technical support team who will be pleased to help you.
 

Title Description  
KASP manual Full user guide for running KASP in your own laboratory Download
KASP quick start guide Outline protocol for set up of KASP genotyping reactions Download 
How does KASP work? Overview of how KASP genotyping chemistry works and how data is scored at LGC Download
How much DNA should I send for my genotyping project? This document outlines the amount of DNA required for a genotyping project run in our service laboratories Download
Reference list Partial list of species that have been genotyped using KASP Download
Trial kit manual User guide for running the KASP genotyping trial kit Download
Trial kit quick start guide Outline protocol for running the KASP genotyping trial kit Download
Validation kit manual User guide for running the KASP Genotyping validation kit (only sent to customers that are using instruments that we are not familiar with). Download
Running KASP on the ABI 7500 User manual for setting up and running KASP on the ABI 7500 qPCR instrument Download
Running KASP on the ABI 7900 User manual for setting up and running KASP on the ABI 7900 qPCR instrument Download
Running KASP on the ABI StepOne and StepOne Plus User manual for setting up and running KASP on the ABI StepOne and StepOne Plus qPCR instrument Download
Running KASP on the ABI Viia7 User manual for setting up and running KASP on the ABI ABI Viia7 qPCR instrument Download
Running KASP on the BIO-RAD CFX User manual for setting up and running KASP on the BIO-RAD CFX qPCR instrument Download
Running KASP on the Roche LC480 User manual for setting up and running KASP on the Roche LC480 qPCR instrument Download
Running KASP on the illumina EcoRT User manual for setting up and running KASP on the illumina EcoRT qPCR instrument Download
Running KASP on the Agilent Mx3000 / Mx3005P User manual for setting up and running KASP on the Agilent Mx3000 / Mx3005P instrument Download
Running KASP on Roche LC96 Factsheet gives detailed explanation of the issues
running KASP genotyping on an LC96 instrument
Download
KASP thermal cycling conditions A document containing details of all the commonly used thermal cycling protocols for KASP genotyping. Download
KASP thermal cycling 61-55ºC touchdown protocol The standard KASP thermal cycling conditions.   Download
KASP thermal cycling 68-62ºC touchdown protocol An alternative KASP thermal cycling protocol, typically used for assays with high %GC. Download
KASP thermal cycling 2-step 57ºC protocol An alternative KASP thermal cycling protocol, typically used for assays with low %GC. Download
KASP thermal cycling 65-57ºC touchdown protocol The standard KASP thermal cycling conditions for the Fluidigm platform. Download
KASP thermal cycling recycling protocol The thermal cycling conditions for further cycling (‘recycling’) of KASP genotyping reactions. Download
Running KASP on Thermo Scientific Piko Real User manual for setting up and running KASP on the Thermo Scientific PikoReal instrument Download