Product info

Simplify your SNP genotyping assays with automated precision at an affordable price.

ValuMix™ assays for SNP genotyping consist of two custom dual-labelled probes and a primer pair delivered at exact quantities in a single tube. These mixes are engineered to provide the highest precision as an optimised blend of primers and probes ready for immediate use in your SNP genotyping assay.

Choose from three probe chemistries: BHQplus™, MGB or LNA Probes. Each probe type is available in three standard sizes and is compatible with a complete set of dye options to ensure optimal performance with your instrument’s optics.

Key benefits:

  • Short probes for high specificity: BHQplus, MGB and LNA Probes increase the melting temperature (Tm) of the probe:target duplex, enabling improved sequence specificity.
  • High signal-to-noise for increased sensitivity: The increased quenching efficiency of BHQplus, MGB and LNA Probes improves sensitivity with lower background noise and a higher signal-to-noise ratio.
  • Improved SNP detection: High duplex stability with a shorter probe leads to enhanced ∆Tm and improved SNP/mismatch discrimination.
  • Save time and money: Reduce pipetting errors and repeat experiments with a ready-to-use optimal blend of high quality probes and primers at an affordable price.
 

 

Probe

5' fluorescent dye

Abs (nm)

Em (nm)

3' quencher

Probe 1   FAM 495 520 BHQ-1 or EDQ*
Probe 2   TET 521 536 BHQ-1 or EDQ
  CAL Fluor Gold 540 522 544 BHQ-1 or EDQ
  CIV-550 530 550 BHQ-1 or EDQ
  HEX 535 556 BHQ-1 or EDQ
  CAL Fluor Orange 560 538 559 BHQ-1 or EDQ

 

* MGB probes come with Eclipse Dark Quencher(EDQ). LNA and BHQplus Probes are available with BHQ-1.

40x or 80x assay mix for SNP genotyping

To order a ValuMix assay for SNP Genotyping as a 40x or 80x assay mix, please include the respective concentration of 8 µM probe and 36 µM primer (40x) or 16 µM probe and 72 µM primer (80x) in the notes field along with your diluent of choice (water, TrisHCl, T10E0.1, or T10E1).

For a 40x and 80x assay mix, we assume 200 nM probe and 900 nM primer as the final reaction concentration. Water is not an option for MGB Probes.

 

Use the spectral overlay tool to view compatible dyes for your instrument. Learn more

 

Choose from one of three duplex stabilising probe chemistries

Duplex stabilising probes enable shorter probe design while maintaining the optimal melting temperature (Tm). The shorter probe improves the signal-to-noise ratio and greatly enhances mismatch discrimination making them ideal for probe-based SNP genotyping.

LGC Biosearch Technologies is the only supplier that offers three duplex stabilising chemistries (BHQplus, MGB and LNA Probes), which gives you flexibility to optimise your assay and maximise performance.

  • BHQplus Probes: BHQplus hydrolysis probes consist of a 5′ fluorescent reporter dye, a 3′ BHQ, and modified C and T nucleotides throughout the probe sequence which are responsible for enhanced probe-target binding stability. The compact sequences of BHQplus Probes are simple to design and typically 15-25 bases in length.
     
  • Minor Groove Binder (MGB) Probes: MGB Probes consist of a 5′ fluorescent reporter dye and a 3′ Eclipse Dark Quencher (EDQ) conjugated to a MGB moiety that stabilises the probe-target duplex. The short and simple 8-30 base MGB Probe design increases the ∆Tm for improved SNP discrimination, particularly when the mismatches are within the minor groove binding region.
     
  • Locked Nucleic Acid (LNA) Probes: LNA hydrolysis probes consist of a 5′ fluorescent reporter dye, a 3′ BHQ, and up to seven LNA bases in the probe sequence. LNAs are modified RNA bases in which the ribose is “locked” with a methylene bridge, fixing it in the C3'-endo conformation. LNA bases have increased affinity for complementary DNA bases, which increases the probe melting temperature and enables a shorter probe design. Each LNA monomer substituted into the 15-25 base probe sequence increases the Tm of the duplex by 2-8 °C allowing for an exact fine-tuning. Order LNA Probes for SNP genotyping.

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Ordering a 40x or 80x Assay Mix

To order your ValuMix for SNP Genotyping as a 40x or 80x assay, please include the respective concentration of 8 µM probe and 36 µM primer (40x) or 16 µM probe and 72 µM primer (80x) in the notes field along with your diluent of choice (water, TrisHCl, T10E0.1, or T10E1). For a 40x or 80x assay mix, we assume 200 nM probe and 900 nM primer as the final reaction concentration. Water is not an option for MGB probes.

(ex. 8 µM probe and 36 µM primer for 40x assay mix, T10E0.1 buffer)

Click here to order LNA probes for SNP genotyping.

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Are you trying to include RNA bases? RNA bases are not allowed in BHQnova probes and so the R character will be treated as a DNA wobble instead (A/G). Please click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in standard BHQplus probes and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in standard MGB probes and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
RNA bases are not allowed in standard Molecular Beacons. Please use the custom oligo form custom oligo form to order these modifications or click here for help.
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Internal modifications are not allowed in standard Molecular Beacons. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
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Internal modifications are not allowed in standard primers. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
No special modifications are allowed in BHQplus® probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
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Inosine modifications must be included in brackets such as [I] or [i].
Internal modifications are not allowed in standard primers. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
No special modifications are allowed in BHQplus® probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
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Probes and primers for this type of ValuMix Assay cannot include internal modifications. Click here for help.
Internal modifications are not allowed in standard MGB probes. For additional assistance, click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in ValuMix assays and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide custom oligonucleotide or click here for help.
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This probe sequence is too long. Consider ordering this sequence as a BHQnova® probe. For additional assistance, click here for help.
This probe sequence exceeds the 30 base limit for ValuMix assays for SNP genotyping. Please order this oligo as a BHQplus® probe or MGB probe BHQplus® probe or MGB probe or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
The internal modification you used in the sequence is not available with this 5' Modification. You need to remove it from your sequence.
Probes and primers for this type of ValuMix Assay cannot include internal modifications. Click here for help.
Internal modifications are not allowed in standard MGB probes. For additional assistance, click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in ValuMix assays and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
An invalid character has been entered into the sequence. For additional assistance, click here for help.
The sequence contains open brackets. Please, verify your sequence.
If you are ordering an LNA please use an excel order form and email to info@biosearchtech.com
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Purification RPC
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Technical specs

 ValuMix Assay for SNP Genotyping
Probe length 8 - 30 bases recommended
Dyes FAM / TET
FAM / CAL Fluor Gold 540
FAM / CIV-550
FAM / HEX
FAM / CAL Fluor Orange 560
Quenchers BHQ-1, EDQ (MGB)
Yield 2 nmol probe / 9 nmol primer 
5 nmol probe / 22.5 nmol primer
12 nmol probe / 54 nmol primer
Purification Probe - Reverse Phase HPLC
Primers - Reverse Phase Cartridge
Delivery format Dry or in solution (water, TrisHCl, T10E0.1, T10E1) *
Quality control MS and UHPLC
Shelf life 12 months from date of manufacture
Shipping Ambient or dry ice (in solution)

 

* MGB probes are not offered in water.

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