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ValuMix SNP genotyping assays

Product info

Simplify your SNP genotyping assays while achieving high precision at an affordable price.

Biosearch Technologies ValuMix SNP Genotyping Assays combine the gold-standard quenching power of the BHQ quencher with the BHQplus™ Probe engineered to provide the highest precision in an optimized blend of primers tailored to your SNP genotyping assay in a single tube.

Why ValuMix?

  • Make genotyping simple – submit your sequences and receive an optimized blend of probes and primers tailored to your assay in a single tube
  • Do your best research – the enhanced affinity of the BHQplus Probe for the target sequence delivers the high-fidelity required for confident genotyping results
  • Save time and money – reduce pipetting errors and repeat experiments with an optimal blend of high quality primers and probes at an affordable price
BHQplus scatterplot

Scatterplot of a BHQplus SNP Assay, resolving the respective genotypes

Looking for more information? View our poster Genotyping with BHQplus Probes for additional details.

ValuMix Assays for SNP genotyping comprises two custom BHQplus Probes and two primers combined within a single tube.

Order now

Ordering a 40x Assay Mix

To order a 40x assay mix, please include the respective concentration of 8 µM probe and 36 µM primer in the notes field along with your diluent of choice (water, TrisHCl, T10E0.1, or T10E1). Please note, water is not an option for MGB probes. For a 40x assay mix, we assume 200 nM probe and 900 nM primer as the final reaction concentration.

(ex. 8 µM probe and 36 µM primer for 40x assay mix, T10E0.1 buffer)

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Oligo set {{$index + 1}}

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ValuProbe
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This sequence is too short. Click here for help.
This sequence is too short. See Technology Overview
This sequence is shorter than recommended. See Technology Overview
This probe sequence is shorter than our recommended criteria for probe length. You may want to consider ordering this sequence as a dual-labeled BHQ probe or BHQplus® probe or MGB probe dual-labeled BHQ probe or BHQplus® probe or MGB probe instead.
You may want to consider ordering an internally quenched probe for sequences 30 bases or longer.
A surcharge has been applied to the price of this probe because the sequence length is longer than 35 bases.
A surcharge has been applied to the price of this probe because the sequence length is longer than 50 bases.
This oligo sequence is too long. Please contact customer service info@biosearchtech.com for a quote or click here for help.
This probe sequence is too long. Consider ordering this sequence as a BHQnova® probe. For additional assistance, click here for help.
Are you trying to order a Molecular Beacon? Click here to continue Click here to continue
If you are ordering an LNA please use an excel order form and email to info@biosearchtech.com
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
The internal modification you used in the sequence is not available with this 5' Modification. You need to remove it from your sequence.
Are you trying to include RNA bases? RNA bases are not allowed in standard BHQ probes and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in BHQnova probes and so the R character will be treated as a DNA wobble instead (A/G). Please click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in standard BHQplus probes and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in standard MGB probes and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
RNA bases are not allowed in standard Molecular Beacons. Please use the custom oligo form custom oligo form to order these modifications or click here for help.
Inosine modifications are not allowed in standard BHQ probes. Please order on the custom oligo form custom oligo form or click here for help.
Inosine modifications are not allowed in standard Nova probes. Please order on the custom oligo form custom oligo form or click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
An invalid character has been entered into the sequence. For additional assistance, click here for help.
Illegal character(s) were entered. Please review your sequence information.
Internal modifications are not allowed in standard Molecular Beacons. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
Internal modifications are not allowed in standard BHQplus probes. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
Internal modifications are not allowed in standard MGB probes. For additional assistance, click here for help.
Internal modifications are not allowed in standard Nova probes. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
The sequence contains open brackets. Please, verify your sequence.
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add
Probe nmol options
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  • {{item.minNmol}} nmol

Purification
Item price TBD
Field required.
This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Inosine modifications must be included in brackets such as [I] or [i].
Internal modifications are not allowed in standard primers. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
No special modifications are allowed in BHQplus® probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
No special modifications are allowed in MGB probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
Invalid character(s) were entered. Please review your sequence information.
Item price
Field required.
This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Inosine modifications must be included in brackets such as [I] or [i].
Internal modifications are not allowed in standard primers. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
No special modifications are allowed in BHQplus® probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
No special modifications are allowed in MGB probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
Invalid character(s) were entered. Please review your sequence information.
Item price
nmol options
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  • {{primerScale.purifications[order.purificationIndex()].minNmol}} nmol
Clear scale

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You are ordering:
Probe catalog #: {{order.probe1.fivePrime.threePrime.item.catalogId}} Primer catalog #: {{order.primer.item.catalogId}} Assay subtotal: {{(order.probe1.fivePrime.threePrime.item.price + order.probe1.fivePrime.threePrime.item.surcharge + order.primer.primer2.price + order.primer.primer1.price)*order.quantity | currency}} Qty another assay
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Assay #{{$index + 1}}

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You need to change the assay name because it has already been used.
Probe 1 name
5' Modification
Field required.
This sequence is too short. Click here for help.
This sequence is too short. See Technology Overview.
This sequence is shorter than recommended. See Technology Overview.
This probe sequence is too long. Consider ordering this sequence as a BHQnova® probe. For additional assistance, click here for help.
This probe sequence is longer than our recommended criteria for probe length. You may want to consider ordering this sequence as a Dual-labeled BHQ® probe Dual-labeled BHQ® probe instead.
This probe sequence exceeds the 30 base limit for ValuMix assays for SNP genotyping. Please order this oligo as a BHQplus® probe or MGB probe BHQplus® probe or MGB probe or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Probes and primers for this type of ValuMix Assay cannot include internal modifications. Click here for help.
Internal modifications are not allowed in standard MGB probes. For additional assistance, click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in ValuMix assays and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide custom oligonucleotide or click here for help.
An invalid character has been entered into the sequence. For additional assistance, click here for help.
The sequence contains open brackets. Please, verify your sequence.
If you are ordering an LNA please use an excel order form and email to info@biosearchtech.com
Field required.
Purification
Probe 2 name
Field required.
Field required.
This sequence is too short. Click here for help.
This sequence is too short. See Technology Overview.
This sequence is shorter than recommended. See Technology Overview.
This probe sequence is longer than our recommended criteria for probe length. You may want to consider ordering this sequence as a Dual-labeled BHQ® probe Dual-labeled BHQ® probe instead.
This probe sequence is too long. Consider ordering this sequence as a BHQnova® probe. For additional assistance, click here for help.
This probe sequence exceeds the 30 base limit for ValuMix assays for SNP genotyping. Please order this oligo as a BHQplus® probe or MGB probe BHQplus® probe or MGB probe or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
The internal modification you used in the sequence is not available with this 5' Modification. You need to remove it from your sequence.
Probes and primers for this type of ValuMix Assay cannot include internal modifications. Click here for help.
Internal modifications are not allowed in standard MGB probes. For additional assistance, click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in ValuMix assays and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
An invalid character has been entered into the sequence. For additional assistance, click here for help.
The sequence contains open brackets. Please, verify your sequence.
If you are ordering an LNA please use an excel order form and email to info@biosearchtech.com
3' Modification
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Probe nmol options
Field required.
  • {{item.minNmol}} nmol

Purification
Item price TBD

Forward primer

Sequence name
Field required.
This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Probes and primers for this type of ValuMix Assay cannot include internal modifications. Click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide custom oligonucleotide or click here for help.
Purification RPC

Reverse primer

Sequence name
Field required.
This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Probes and primers for this type of ValuMix Assay cannot include internal modifications. Click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide custom oligonucleotide or click here for help.
Purification RPC
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You are ordering:
Probe catalog #: {{order.probe2.fivePrime.threePrime.item.catalogId}} Assay subtotal: {{(order.probe2.fivePrime.threePrime.item.price)*order.quantity | currency}} Qty another assay
Max assay forms reached. Please add current assays to your cart, then return to form to continue adding more assays.
Note

Subtotal: {{ctrl.getTotalPrice() | currency}} add to cart add to cart

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