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High-throughput, high-quality RNA purification for COVID-19 detection

COVID-19 testing protocols require fast, reliable, high-yield, high-quality and easily automatable, sample preparation methods. sbeadex™ viral RNA purification kits meet these requirements. For Research Use Only. Not for use in diagnostic procedures.

Key benefits

  • Qualified protocol for RUO COVID-19 assays  (qualified for research use only using SeraCare’s AccuPlex™ reference material)
  • Compatible with most popular robotic platforms, including the Kingfisher™ and our high-throughput oKtopure™ instrument
  • Fast – both short (25 min) and standard (45 min) protocols available
  • High sensitivity: 750 copies in 1 mL; 100% recovery rate
  • High-throughput batch sizes (5,000 purifications)
  • Water-based wash buffers reduce solvent carryover, resulting in absence of PCR inhibitors in final eluate
  • High-RNA-quality for all downstream applications including qPCR and PCR
Product name Item name Number of purifications per kit  
sbeadex viral RNA purification kit NAP-40-024-02 960 Order now
NAP-40-024-03 5000 
NAP-40-024-04 10000 
sbeadex viral RNA purification kit, no proteinase K NAP-40-026-02 960 Order now
NAP-40-026-03 5000 
NAP-40-026-04 10000

We offer a no dangerous goods option and a no dangerous goods with no proteinase K option for Asia Pacific.

Product info

sbeadex viral RNA purification kits are magnetic-bead based kits are validated for research use only using AccuPlex™ reference material (SeraCare), replicating a real-life clinical sample as far as practically possible, and are compatible with Biosearch Technologies’ COVID-19 detection kit. The sbeadex viral RNA isolation kit portfolio offers products for all scales of nucleic acid preparation from buccal swab and sputum samples, eluting into 100 µL buffer.

RNA preparation protocol

sbeadex beads allow efficient and high quality purification of small and large DNA fragments. The beads are double coated, resulting in a unique dual binding protocol that allows elution into water, reducing solvent carryover:

  sbeadex viral RNA purification kit Competitor A
  N Average Cq StDev Average Cq StDev
Clean up sample 12 30.35 0.14 33.80 0.15
Direct spike-in sample 3 31.00 0.44 33.80 0.12

Figure 1:  Demonstration of no loss of RNA during the sbeadex viral RNA purification kit protocol.  500 copies of a 2019-COVID artificial RNA reference material were added to a sample (nasopharyngeal swab) and the sample processed with the sbeadex viral RNA purification kit. SARS-CoV-2 was detected in this preparation and a directly spiked positive control sample via a RT-qPCR at two separate Biosearch Technologies sites (reaction components described below). The concordant Cq values from both samples demonstrate no loss of sample due to the extraction step.

Recommended products

RT qPCR reagents* including master mix and enzyme for SARS-CoV-2 assay development are also available

Item name  
RapiDxFire 1-step RT-qPCR System View product
QuickExtract™  RNA Kit View product
QuickExtract™ DNA Kit View product

Probes and primers that target SARS-CoV-2

Item name  
2019-nCoV CDC-qualified Probe and Primer Kits for SARS-CoV-2 View product
US CDC 2019-nCoV ValuPanel Reagents* View product
Charité 2019-nCoV BHQ ValuPanel Reagents* View product
Charité 2019-nCoV BBQ-650 ValuPanel Reagents* View product
Yale 2019-nCoV SalivaDirect ValuPanel Reagents* View product

For a complete SARS-CoV-2 Quality Solutions, consider the AccuPlex™ SARS-CoV-2 Verification Panel coupled with the AccuPlex™ SARS-CoV-2 Reference Material Kit from LGC SeraCare Life Sciences. The target sequence of interest is packaged into a stable non-infectious, and replication-deficient alphavirus particle and the products contain positive materials directed against the published CDC and WHO consensus sequences.

What do you require to develop your SARS-CoV-2 assay?

We offer probes and primers specific to Coronavirus as well as enzymes, master mix, and extraction capabilities.

Let’s discuss your needs

*For Research Use Only. Not for use in diagnostic procedures.