IntelliQube™ Liquid Handling
IntelliQube leverages best-in-class, multi-functional dispensing for high-speed liquid handling in 384- or 768-well Array Tape™. Precise sample dispensing into Array Tape is handled by an interchangeable CyBio® FeliX 96- or 384-channel pipetting head. Reagent dispensing is performed by the Dispense Jet, a 4-channel, non-contact dispense head that loads the assay mixtures required for your protocols. With CVs less than 5%, the liquid handling on the IntelliQube offers exceptional reproducibility and data quality.
The IntelliQube Pipette Wash system reduces tip waste and associated consumable costs. The Pipette Wash uses fluid agitation around the dispense tips to ensure complete interior and exterior cleaning of the tips, while a touch-off feature is used to counteract surface tension and remove residual droplets. Complete elimination of the wash basin contents after each wash cycle combined with the ability to incorporate a wash additive, such as bleach, ensures testing integrity and mitigates the risk of cross-contamination.
Liquid Handling Specifications
||Sample Dispensing CyBio® FeLiX pipette head
||Assay Dispensing Dispense Jet
||Single jet solenoid valve
||96- or 384-channel
||Nucleic acid templates
|Source plate positions
||10 (ambient temperature)
||3 (temperature controlled)
|Supported source plates
||ANSI/SBS compliant 96/384-well formats ≤15 mm in height
||ANSI/SBS compliant 96-well formats ≤ 30 mm in height
|Total dispensing time
||Approximately 5 to 6 minutes per array
Users can expect minimal well-to-well variability in Array Tape, providing The IntelliQube demonstrated a total standard deviation (SD) of 0.087 cycles when 768 replicate wells each containing 2048 copies of a NIST calibrated human – gDNA reference standard were analyzed for a non- transcribed locus in hgDNA.
Figure 1. Cq Uniformity <0.09 SD
The IntelliQube can support assays across a large range of input concentrations while maintaining data integrity. When analyzing a 1000 bp synthetic target serially diluted across eight orders of magnitude, the IntelliQube demonstrated a linear dynamic range of quantifications between 4x107 to 40 copies/reaction.
Figure 2. Linear dynamic range of quantification from 4x107 to 40 copies/reaction
Minimal differences in target quality can be accurately resolved between reaction wells on the IntelliQube. Using NIST calibrated hgDNA reference standard, reactions were prepared to represent 1.2 fold differences in template concentration. When analyzed for a non-transcribed locus in hgDNA, the IntelliQube demonstrated the ability to resolve 1.2 fold differences with greater than 97% sensitivity and specificity.
Figure 3. 1.2 fold resolution
The IntelliQube provides the sensitivity necessary to amplify and detect even 1-2 copies of target DNA in a reaction well. Although the theoretical limit of detection (LOD) provided by Poisson statistics is limited to three molecules, IntelliQube has demonstrated the ability to detect as little as 1-2 molecules of hgDNA per reaction.
IntelliQube Performance Specifications
|Dimensions (W x D x H)
||49" x 34" x 75" (with cart)
||120/240 VAC, 15/7.5 A
||20-80% Relative Humidity, non-condensing
||80-100 psi, bursts up to 15 SCFM
||Reverse Osmosis or Deionized
||Conforms to the European Machinery Directive (2006/42/EC) and the EMC Directive (2004/108/EC). For Research Use Only. Not for use in Diagnostic Procedures.
|Estimated End-point PCR Throughput Per 8 Hours (With Hydrocycler2)
||Sixty Five 384-well arrays
24,960 data points
||Peltier block type
||384/768-well Array Tape
||20 - 100°C
||±0.5°C at 60°C
|Heating ramp rate
|Excitation filter (nm)
|Emission Filter (nm)
|Dyes with expected compatability
Cal Fluor® Red 590
Cal Fluor® Red 610