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ROX passive reference dye is used to normalize for non-PCR related fluorescence signal variation on certain real-time thermocyclers (ie., Applied Biosystems Prism™ 7000, 7700 and 7900 instruments). The traditional ROX passive reference dye does not take part in the PCR reaction and its fluorescence should remain constant during the PCR reaction. However, under certain assay conditions the ROX signal decreases over time and can complicate subsequent quantification of results. SuperROX is a highly stable ROX passive reference dye that completely eliminates ROX drop and can be used when a water soluble version of ROX is required as a passive reference for normalization of fluorescent signals in real-time PCR.

Properties:

  • Absorption Maximum (Lambda Max):

    585

  • Extinction Coefficient at Lambda max:

    113000

  • Fluorescence Maximum:

    606

Product usage:

    SuperROX is supplied at a 50X concentration (approx. 15 µmolar) in HPLC grade water. For the ABI prism 7000 dilute the SuperROX 50-fold. For the ABI Prism 7700 dilute the SuperROX 167-fold.

Storage and handling:

  • Shipping conditions:

    Ambient

  • Storage conditions:

    +2 to +8 °C, dark