Stellaris® FISH Probes, eGFP with Quasar® 570 Dye
Stellaris® FISH Probes, eGFP with Quasar® 570 Dye consists of a set of dye-labeled oligos mixed and pooled into a final delivered amount of 5 nmol, which yields approximately 200-400 hybridizations under standard conditions. Designed to detect eGFP transcripts in specimens using fluorescence in situ hybridization (FISH).
Design Criteria: Product was designed against enhanced Green Fluorescent Protein eGFP a.k.a. eGFP; GFP (NCBI gene ID:N/A) and nucleotides 289-1005 of U76561. This is an inclusive probe set designed to also detect: pCMS eGFP Clontech PT3268-5pEF eGFP Addgene Plasmid 11154pCEFL eGFP Addgene 38121pME/pE eGFP Addgene 36410pRSET-emGFP Invitrogen V353-20eYFPeCFPBFP (invitrogen)mTurqoise. This design has been tested in cell culture and frozen mouse brain tissue using Quasar 570 dye-labeled probes and found to produce the expected signal. The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.
- Absorption Maximum (Lambda Max):
- Fluorescence Maximum:
- Additional information:
- Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.
For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.
Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols.
Storage and handling:
- Storage and handling:
- Storage conditions:
- +2 to +8 °C
Biosearch, the Biosearch Technologies logo, Black Hole Quencher, BHQ, BHQplus, CAL Fluor, Quasar, Pulsar, Stellaris and SuperROX are registered trademarks of Biosearch Technologies, Inc.
Permitted Product Usage
All products offered for sale by Biosearch Technologies, Inc. (BTI) are sold under the condition that they be used for research purposes only and are prohibited from use in diagnostic or other commercial applications unless explicitly authorized by separate written agreement with BTI.
Black Hole Quencher® (BHQ), CAL Fluor®, Quasar® and Pulsar® dyes (referred to collectively as “BTI Dyes”) are sold to the purchaser only for internal R&D use, and are not to be used for clinical or diagnostic purposes. Neither the BTI Dyes nor the compounds synthesized with them are to be re-packaged or re-sold. Separate licenses to the BTI Dyes may be available for applications other than the aforementioned internal R&D. Please inquire via email@example.com.
The Black Hole Quencher dye technology is protected in the United States and other countries by U.S. patents and continuations numbered 7,019,129, 7,019,129B1, 7,109,312B2, 7,582,432, 8,410,255B2 and 8,440,399B2 issued to Biosearch Technologies, Inc. The CAL Fluor technology is covered by U.S. patent number 7,344,701B2. The Quasar technology is covered by U.S. Patent numbers 7,705,150B2, 7,868, 157B2 and 8,436,153B2. The Pulsar technology is covered by U.S. Patent numbers 7,635,762B2 and 8,119,781B2.
Supercolumns are protected by U.S. Patent 6,761,855 B1, "Column for solid phase processing" issued to Biosearch Technologies, Inc. Contact firstname.lastname@example.org for more information. Supercolumns for use on ABI 3900 or equivalent rotary cartridge style shoulder mount design are also manufactured under license from McLuen Design, U.S. Patents 8,158,085 and 8,404,196.
Stellaris products are sold under license from Rutgers University and may be used under its patent rights for Research Use Only.
Information on licensing programs for the commercial and/or diagnostic use of these products is available by contacting email@example.com.
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