Stellaris DesignReady Professionaly Designed Probe Sets

DesignReady Stellaris™ RNA FISH Probe Sets take the guesswork out of designing your own custom assay. These professionally designed sets go through rigorous pre- and post-design bioinformatic analysis to maximize specificity and most are available in the following dyes: CAL Fluor™ Red 590, Quasar™ 570, CAL Fluor Red 610, Quasar 670, CAL Fluor Red 635, CAL Fluor Orange 560 and FAM.

Enter your RNA target into the search filter below to determine whether there is a DesignReady probe set for your target or whether you will need to design a new probe set using our Custom Stellaris probe design software.

Price	Size/Scale	Note	Qty	Add	Conjugation ratio
${{item.salePrice}}	${{item.userPriceBook.salePrice}}	${{item.unitPrice}}	${{item.userPriceBook.unitPrice}}	TBD	{{item.sizeAndScale}}	{{item.message}}

Product information: One set of Stellaris FISH probes contain a blend of up to 48 oligos labeled with a fluorophore. Probes arrive lyophilised and ready to use.

Delivered Amount: 5 nmol of pooled oligos

Turnaround time: Stellaris DesignReady Probe Sets ship in 5 to 15 business days. A more precise shipping estimate will be given upon quotation or order.

Dye Selection: Please visit our Stellaris Dyes and Modifications Page to learn about our dyes and modifications. Due to the variable autofluorescence in the green channel inherent to some cells and tissues, it is not recommended to use FAM for some Stellaris RNA FISH applications. We recommend testing your sample for green channel autofluorescence before ordering FAM labeled sets or ordering an alternative dye that is compatible with your experiment.

Storage Guidelines: Stellaris RNA FISH Probes are shipped dry at ambient temperature and can be stored at +2 to +8 °C in this state. Dissolved probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use of dissolved probe mix, storage at +2 to +8 °C in the dark for up to a month is recommended. For storage lasting longer than a month, we recommend aliquoting and freezing probes in the dark at -15 to -30 °C.

Reconstituting the dried probe stock: A DesignReady Probe Set can provide up to 400 hybridizations. Re-dissolve the dried oligonucleotide probe blend in 400 μL of TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) to create a probe stock of 12.5 μM. Mix well by pipetting up and down, and then vortex and centrifuge briefly.

Note: When performing Stellaris RNA FISH, it is imperative to limit RNA degradation. Ensure that all consumables and reagents are RNase-free. Recipe above is for set volumes. Please adjust accordingly.

Protocols: Please visit our Stellaris RNA FISH Protocols page.

Absorption and emission spectral charts: Please visit our Calibration dyes page.

You can find the most up-to-date protocols for Stellaris RNA FISH Probes by visiting the Stellaris RNA FISH Protocols page. For answers to specific questions please visit our Stellaris RNA FISH FAQ section. The protocols and FAQs apply to imaging on a widefield fluorescence microscope with at least a 60X-oil, 1.4 NA objective, mercury or metal halide lamps, and cooled CCD camera. We do not recommend confocal applications as an entry point, although we have many successful experienced confocal users.

See Stellaris RNA FISH Probes at work by viewing our Stellaris image gallery. Images are contributed by customers and by scientists from LGC Biosearch Technologies.

Comprehensive Stellaris Education and Links Page

Dyes and Modifications for Stellaris

Stellaris Citation Center

Stellaris Getting Started Guide

Related products

The following peer-reviewed customer publications cite Stellaris RNA FISH products and/or services. Over 33% of publications citing Stellaris RNA FISH publish in Cell, Nature, or Science speaking volumes about the quality of our product and the thought leadership of our customers.

424

Publications

148

Nature, Cell or Science

How to Cite Stellaris RNA FISH

The following Publications include citations of all Stellaris® RNA FISH product types.

Stellaris Citations Papers labeled as Nature, Science, or Cell include sub-groups of these publishing groups such as communications, letters, methods etc.
*Updated August 2016

Stellaris Scoop Research Highlights

Stellaris® RNA FISH DesignReady probe sets take the guesswork out of designing custom assays

Programmable RNA Tracking in Live Cells with CRISPR/Cas9
Nelles et al. Cell. 2016.

Stellaris® RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature Long Noncoding RNAs in Adherent Cells
Orjalo et al. Methods in Molecular Biology. 2016.

Simultaneous Detection of Nuclear and Cytoplasmic RNA Variants Utilizing Stellaris® RNA Fluorescence In Situ Hybridization in Adherent Cells
Coassin et al. Methods in Molecular Biology. 2014.

Published using professionally designed DesignReady probe sets.

Comprehensive Publication List

DesignReady Probe Sets

RNA FISH DesignReady Product Page

Browse All DesignReady Probe Sets

Catalog Number	Gene Name and Organism	Associated Publications
VSMF-1014-5	eGFP	CD133 does not enrich for the stem cell activity in vivo in adult mouse prostates Weiet al. Stem Cell Research. 2016. url: https://dx.doi.org/10.1016/j.scr.2016.03.003
VSMF-2415-5	Human TFRC	High Impact Programmable RNA Tracking in Live Cells with CRISPR/Cas9 Nelleset al. Cell. 2016. url: https://dx.doi.org/10.1016/j.cell.2016.02.054
VSMF-2003-5	Human ACTB	High Impact Programmable RNA Tracking in Live Cells with CRISPR/Cas9 Nelleset al. Cell. 2016. url: https://dx.doi.org/10.1016/j.cell.2016.02.054
VSMF-2162-5	Human H19	High Impact Stellaris® RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature Long Noncoding RNAs in Adherent Cells Orjalo et al. Methods in Molecular Biology 1402 Springer Protocols. 2016. url: https://dx.doi.org/10.1007/978-1-4939-3378-5_10
VSMF-2161-5	Human H19	Visualizing allele-specific expression in single cells reveals epigenetic mosaicism in an H19 loss-of-imprinting mutant Ginartet al. Genes and Development. 2016. url: https://dx.doi.org/10.1101/gad.275958.115.
VSMF-2230-5	Human MYC	High Impact Stellaris® RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature Long Noncoding RNAs in Adherent Cells Orjalo et al. Methods in Molecular Biology 1402 Springer Protocols. 2016. url: https://dx.doi.org/10.1007/978-1-4939-3378-5_10
VSMF-2307-5	Human PVT1	High Impact Stellaris® RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature Long Noncoding RNAs in Adherent Cells Orjalo et al. Methods in Molecular Biology 1402 Springer Protocols. 2016. url: https://dx.doi.org/10.1007/978-1-4939-3378-5_10
VSMF-2430-5	Human XIST	High Impact Stellaris® RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature Long Noncoding RNAs in Adherent Cells Orjalo et al. Methods in Molecular Biology 1402 Springer Protocols. 2016. url: https://dx.doi.org/10.1007/978-1-4939-3378-5_10
VSMF-2448-5	Human CCNA2	High Impact Programmable RNA Tracking in Live Cells with CRISPR/Cas9 Nelleset al. Cell. 2016. url: https://dx.doi.org/10.1016/j.cell.2016.02.054
VSMF-1019-5	GFP	Synonymous modification results in highfidelity gene expression of repetitive protein and nucleotide sequences Wu et al. Genes & Development. 2015. url: https://dx.doi.org/10.1101/gad.259358.115
VSMF-2250-5	Human NEAT1 middle segment	Prion-like domains in RNA binding proteins are essential for building subnuclear paraspeckles Hennig et al. The Journal of Cell Biology. 2015. url: https://dx.doi.org/10.1083/jcb.201504117
VSMF-2054-5	Human CDKN1A	High Impact Simultaneous Detection of Nuclear and Cytoplasmic RNA Variants Utilizing Stellaris ® RNA Fluorescence In Situ Hybridization in Adherent Cells Coassin et al. Methods in Molecular Biology. 2014. url: https://dx.doi.org/10.1007/978-1-4939-1459-3_15
VSMF-2211-5	Human MALAT1	High Impact Simultaneous Detection of Nuclear and Cytoplasmic RNA Variants Utilizing Stellaris ® RNA Fluorescence In Situ Hybridization in Adherent Cells Coassin et al. Methods in Molecular Biology. 2014. url: https://dx.doi.org/10.1007/978-1-4939-1459-3_15
VSMF-2102-5	Human ERBB2	Super-resolution Second Harmonic Super-resolution Microscopy for Quantification of mRNA at Single Copy Sensitivity Liu et al. ACS Nano. 2014. url: https://dx.doi.org/10.1021/nn505096t
VSMF-1011-5	dsRED2	Coactivators enable glucocorticoid receptor recruitment to fine-tune estrogen receptor transcriptional responses Bolt et al. Nucleic Acids Research. 2013. url: https://dx.doi.org/10.1093/nar/gkt100

Stellaris DesignReady Professionaly Designed Probe Sets

Related products

424

Publications

148

Nature, Cell or Science

Research Focus

Organism

Application

RNA Target Type

Multiplexing

Publications citing DesignReady Stellaris Probe Sets

Dyes

Stellaris Scoop Research Highlights

DesignReady Probe Sets

RNA FISH DesignReady Product Page

CD133 does not enrich for the stem cell activity in vivo in adult mouse prostates

Programmable RNA Tracking in Live Cells with CRISPR/Cas9

Programmable RNA Tracking in Live Cells with CRISPR/Cas9

Stellaris® RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature Long Noncoding RNAs in Adherent Cells

Visualizing allele-specific expression in single cells reveals epigenetic mosaicism in an H19 loss-of-imprinting mutant

Stellaris® RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature Long Noncoding RNAs in Adherent Cells

Stellaris® RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature Long Noncoding RNAs in Adherent Cells

Stellaris® RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature Long Noncoding RNAs in Adherent Cells

Programmable RNA Tracking in Live Cells with CRISPR/Cas9

Synonymous modification results in highfidelity gene expression of repetitive protein and nucleotide sequences

Prion-like domains in RNA binding proteins are essential for building subnuclear paraspeckles

Simultaneous Detection of Nuclear and Cytoplasmic RNA Variants Utilizing Stellaris ® RNA Fluorescence In Situ Hybridization in Adherent Cells

Simultaneous Detection of Nuclear and Cytoplasmic RNA Variants Utilizing Stellaris ® RNA Fluorescence In Situ Hybridization in Adherent Cells

Second Harmonic Super-resolution Microscopy for Quantification of mRNA at Single Copy Sensitivity

Coactivators enable glucocorticoid receptor recruitment to fine-tune estrogen receptor transcriptional responses

Research Focus

Development

Memory of cell shape biases stochastic fate decision-making despite mitotic rounding

Developmental programming modulates olfactory behavior in C. elegans via endogenous RNAi pathways

The Wnt pathway limits BMP signaling outside of the germline stem cell niche in Drosophila ovaries

A system to study mechanisms of neuromuscular junction development and maintenance

Clustered nuclei maintain autonomy and nucleocytoplasmic ratio control in a syncytium

A simple and rapid method for combining fluorescent in situ RNA hybridization (FISH) and immunofluorescence in the C. elegans germline

Topologically associated domains enriched for lineage specific genes reveal expression-dependent nuclear topologies during myogenesis

Single-cell differences in matrix gene expression do not predict matrix deposition

Visualizing allele-specific expression in single cells reveals epigenetic mosaicism in an H19 loss-of-imprinting mutant

Overlapping cell population expression profiling and regulatory inference in C. elegans

Mutagenesis of GATA motifs controlling the endoderm regulator elt-2 reveals distinct dominant and secondary cis-regulatory elements

Germ Plasm Biogenesis—An Oskar-Centric Perspective

RNase P protein subunit Rpp29 represses histone H3.3 nucleosome deposition

Brain regions and molecular pathways responding to food reward type and value in honey bees

Unequal distribution of 16S mtrRNA at the 2-cell stage regulates cell lineage allocations in mouse embryos

Automated detection and quantification of single RNAs at cellular resolution in zebrafish embryos

LncRNA-HIT Functions as an Epigenetic Regulator of Chondrogenesis through Its Recruitment of p100/CBP Complexes

The primate-specific noncoding RNA HPAT5 regulates pluripotency during human preimplantation development and nuclear reprogramming

Single-cell messenger RNA sequencing reveals rare intestinal cell types

Antagonist Xist and Tsix co-transcription during mouse oogenesis and maternal Xist expression during pre-implantation development calls into question the nature of the maternal imprint on the X chromosome

Analysis of dynamic changes in retinoid-induced transcription and epigenetic profiles of murine Hox clusters in ES cells

piRNA-guided slicing of transposon transcripts enforces their transcriptional silencing via specifying the nuclear piRNA repertoire

MED GATA factors promote robust development of the C. elegans endoderm

Single Cell Analysis Reveals Concomitant Transcription of Pluripotent and Lineage Markers During the Early Steps of Differentiation of Embryonic Stem Cells

G1/S Inhibitors and the SWI/SNF Complex Control Cell-Cycle Exit during Muscle Differentiation

Robust hematopoietic progenitor cell commitment in the presence of a conflicting cue

DAZL regulates Tet1 translation in murine embryonic stem cells

An alternative pluripotent state confers interspecies chimaeric competency

Single-molecule analysis of myocyte differentiation reveals bimodal lineage commitment†

Asymmetric Transcript Discovery by RNA-seq in C. elegans Blastomeres Identifies neg-1, a Gene Important for Anterior Morphogenesis

CSR-1 and P granules suppress sperm-specific transcription in the C. elegans germline

Voxel-based analysis of the immediate early gene, c-jun, in the honey bee brain after a sucrose stimulus

Single-Cell XIST Expression in Human Preimplantation Embryos and Newly Reprogrammed Female Induced Pluripotent Stem Cells

Licensing of Primordial Germ Cells for Gametogenesis Depends on Genital Ridge Signaling

The Bicoid Class Homeodomain Factors ceh-36/OTX and unc-30/PITX Cooperate in C. elegans Embryonic Progenitor Cells to Regulate Robust Development

Multi-Scale Imaging and Informatics Pipeline for In Situ Pluripotent Stem Cell Analysis

Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically-disordered proteins in C. elegans

Novel Small Leucine-Rich Protein Chondroadherin-like (CHADL) is Expressed in Cartilage and Modulates Chondrocyte Differentiation*

A Predictive Model of Bifunctional Transcription Factor Signaling during Embryonic Tissue Patterning

Stochastic promoter activation affects Nanog expression variability in mouse embryonic stem cells

Chd1 is essential for the high transcriptional output and rapid growth of the mouse epiblast

Quantification of pre-mRNA escape rate and synergy in splicing

Cell Intrinsic Modulation of Wnt Signaling Controls Neuroblast Migration in C. elegans

The long noncoding RNA Neat1 is required for mammary gland development and lactation

Rejuvenation of Meiotic Cohesion in Oocytes during Prophase I Is Required for Chiasma Maintenance and Accurate Chromosome Segregation

A Gene Regulatory Network Controls the Binary Fate Decision of Rod and Bipolar Cells in the Vertebrate Retina

SUMV-1 antagonizes the activity of synthetic multivulva genes in Caenorhabditis elegans