Product information: One set of Stellaris® FISH probes contain a blend of up to 48 oligos labeled with a fluorophore. Probes arrive lyophilized and ready to use.
Delivered Amount: 5 nmol of pooled oligos
Turnaround time: Stellaris DesignReady Probe Sets ship in 5 to 15 business days. A more precise shipping estimate will be given upon quotation or order.
Dye Selection: Please visit our Stellaris Dyes and Modifications Page to learn about our dyes and modifications. Due to the variable autofluorescence in the green channel inherent to some cells and tissues, it is not recommended to use FAM for some Stellaris RNA FISH applications. We recommend testing your sample for green channel autofluorescence before ordering FAM labeled sets or ordering an alternative dye that is compatible with your experiment.
Storage Guidelines: Stellaris RNA FISH Probes are shipped dry at ambient temperature and can be stored at +2 to +8 °C in this state. Dissolved probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use of dissolved probe mix, storage at +2 to +8 °C in the dark for up to a month is recommended. For storage lasting longer than a month, we recommend aliquoting and freezing probes in the dark at -15 to -30 °C.
Reconstituting the dried probe stock: A DesignReady Probe Set can provide up to 400 hybridizations. Re-dissolve the dried oligonucleotide probe blend in 400 μL of TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) to create a probe stock of 12.5 μM. Mix well by pipetting up and down, and then vortex and centrifuge briefly.
Note I: When performing Stellaris RNA FISH, it is imperative to limit RNA degradation. Ensure that all consumables and reagents are RNase-free. Recipe above is for set volumes. Please adjust accordingly.
Note II: DesignReady Probe Sets are inclusive probe sets, designed to detect all or most transcript variants for the RNA target listed on its product page. If you are interested in specific splice variants, we recommend that you design a Custom Probe Set or consider our Professional Design Service. Additionally, if there is a closely related species or transcript that is not offered as a DesignReady product, we recommend only using these sets if there is high percentage of sequence homology between the two species or transcripts. Please contact us at email@example.com if you are interested in the Professional Design Service.
Protocols: Please visit our Stellaris RNA FISH Protocols page.
Absorption and emission spectral charts: Please visit our Calibration dyes page.