Stellaris® FISH Probes, Human POLR2A with Quasar® 570 Dye
Human POLR2A consists of a set of Quasar® 570-labeled oligos mixed at equal ratios and pooled into a final delivered amount of 1 nmol, which yields approximately 80 hybridizations under standard conditions. Designed to detect POLR2A transcripts in Human specimens using fluorescence in situ hybridization (FISH).
Design Criteria: Product was designed against Human Polymerase (RNA) II (DNA directed) polypeptide A, 220kD, POLR2A, a.k.a. RPB1; RPO2; POLR2; POLRA; RPOL2 (NCBI gene ID: 5430), and the coding sequence of NM_000937.4 nts 387-6299.The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.
Representative image of Human POLR2A mRNAs detected with a Quasar 570 dye labeled probe set in A549 cells
- Absorption Maximum (Lambda Max):
- Fluorescence Maximum:
- Additional information:
- Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.
For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.
Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols.
Storage and handling:
- Storage conditions:
- Stellaris FISH Probes are shipped dry and may be stored at +2 to +8ºC in this state.
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Permitted Product Usage
All products offered for sale by Biosearch Technologies, Inc. (BTI) are sold under the condition that they be used for research purposes only and are prohibited from use in diagnostic or other commercial applications unless explicitly authorized by separate written agreement with BTI.
Black Hole Quencher® (BHQ), CAL Fluor®, Quasar® and Pulsar® dyes (referred to collectively as “BTI Dyes”) are sold to the purchaser only for internal R&D use, and are not to be used for clinical or diagnostic purposes. Neither the BTI Dyes nor the compounds synthesized with them are to be re-packaged or re-sold. Separate licenses to the BTI Dyes may be available for applications other than the aforementioned internal R&D. Please inquire via email@example.com.
The Black Hole Quencher dye technology is protected in the United States and other countries by U.S. patents and continuations numbered 7,019,129, 7,019,129B1, 7,109,312B2, 7,582,432, 8,410,255B2 and 8,440,399B2 issued to Biosearch Technologies, Inc. The CAL Fluor technology is covered by U.S. patent number 7,344,701B2. The Quasar technology is covered by U.S. Patent numbers 7,705,150B2, 7,868, 157B2 and 8,436,153B2. The Pulsar technology is covered by U.S. Patent numbers 7,635,762B2 and 8,119,781B2.
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Stellaris products are sold under license from Rutgers University and may be used under its patent rights for Research Use Only.
Information on licensing programs for the commercial and/or diagnostic use of these products is available by contacting email@example.com.
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