Stellaris® FISH Probes, Yeast RPO21 with Quasar® 570 Dye
Yeast RPO21 consists of a set of Quasar® 570-labeled oligos at equal ratios and pooled into a final delivered amount of 1 nmol, which yields approximately 80 hybridizations under standard conditions. Designed to detect RPO21 transcripts in specimens using fluorescence in situ hybridization (FISH).
Design Criteria: Product was designed against RNA polymerase II core subunit RPO21 a.k.a. RPB1; RPB220; SUA8 (NCBI gene ID:853395) and nucleotides 1-5202 of NM_ 001180200.1. This is an exclusive probe set designed to detect the following variants: RPO21 NM_001180200.1. The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.
Representative image of yeast RPO21 RNAs detected with a Quasar 570 dye labeled probe set in BY4741 cells.
- Absorption Maximum (Lambda Max):
- Fluorescence Maximum:
- Additional information:
- Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.
For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.
Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols.
Storage and handling:
- Storage and handling:
- Storage conditions:
- +2 to +8 °C
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Permitted Product Usage
All products offered for sale by Biosearch Technologies, Inc. (BTI) are sold under the condition that they be used for research purposes only and are prohibited from use in diagnostic or other commercial applications unless explicitly authorized by separate written agreement with BTI.
Black Hole Quencher® (BHQ), CAL Fluor®, Quasar® and Pulsar® dyes (referred to collectively as “BTI Dyes”) are sold to the purchaser only for internal R&D use, and are not to be used for clinical or diagnostic purposes. Neither the BTI Dyes nor the compounds synthesized with them are to be re-packaged or re-sold. Separate licenses to the BTI Dyes may be available for applications other than the aforementioned internal R&D. Please inquire via firstname.lastname@example.org.
The Black Hole Quencher dye technology is protected in the United States and other countries by U.S. patents and continuations numbered 7,019,129, 7,019,129B1, 7,109,312B2, 7,582,432, 8,410,255B2 and 8,440,399B2 issued to Biosearch Technologies, Inc. The CAL Fluor technology is covered by U.S. patent number 7,344,701B2. The Quasar technology is covered by U.S. Patent numbers 7,705,150B2, 7,868, 157B2 and 8,436,153B2. The Pulsar technology is covered by U.S. Patent numbers 7,635,762B2 and 8,119,781B2.
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