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Yeast RPO21 consists of a set of Quasar® 570-labeled oligos at equal ratios and pooled into a final delivered amount of 1 nmol, which yields approximately 80 hybridizations under standard conditions. Designed to detect RPO21 transcripts in specimens using fluorescence in situ hybridization (FISH). Design Criteria: Product was designed against RNA polymerase II core subunit RPO21 a.k.a. RPB1; RPB220; SUA8 (NCBI gene ID:853395) and nucleotides 1-5202 of NM_ 001180200.1. This is an exclusive probe set designed to detect the following variants: RPO21 NM_001180200.1. The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species. Representative image of yeast RPO21 RNAs detected with a Quasar 570 dye labeled probe set in BY4741 cells.
Properties:
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Absorption Maximum (Lambda Max):
548
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Fluorescence Maximum:
566
Product usage:
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Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.
For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.
Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols.
Storage and handling:
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Shipping conditions:
Ambient
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Storage conditions:
+2 to +8 °C
