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"Yeast TDH1_2_3 consists of a set of Quasar® 570-labeled oligos at equal ratios and pooled into a final delivered amount of 1 nmol, which yields approximately 80 hybridizations under standard conditions. Designed to detect TDH1, TDH2, and TDH3 transcripts in specimens using fluorescence in situ hybridization (FISH).
Design Criteria: Product was designed against glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) TDH1, glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) TDH2, glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) TDH3 a.k.a. GLD1; GLD2; GLD3; HSP35; SSS2 (NCBI gene ID:853395; 853465; 853106) and nucleotides 1-999 of NM_ 0011801321.3.1. This is an inclusive probe set designed to detect the following variants: NM_001181321.3, NM_001181485.3, NM_001181666.1. The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.
Representative image of yeast TDH1_2_3 RNAs detected with a Quasar 570 dye labeled probe set in BY4741 cells."
Properties:
-
Absorption Maximum (Lambda Max):
548
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Fluorescence Maximum:
566
Product usage:
-
"Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.
For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.
Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols."
Storage and handling:
-
Shipping conditions:
Ambient
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Storage conditions:
+2 to +8 °C
