Genotyping by Sequencing (GBS) is the cost effective method of choice for genome wide SNP discovery without prior knowledge of the genome sequence.

Genotyping by Sequencing (GBS), also known as Sequence-Based Genotyping (SBG), has become the method of choice for high throughput discovery of SNPs and simultaneous genotyping in multiple DNA samples. It combines restriction enzyme-mediated complexity reduction with the high-throughput sequencing capacity of Illumina platforms to score random markers across an entire genome

LGCs sample preparation and sequencing experts provide unrivalled support and assist in ensuring the optimal protocol is selected for your application. With expertise in techniques ranging from normalized GBS (nGBS), double digest RadSeq (ddRAD) and other GBS or targeted sequencing based approaches.

LGC has secured a license for the commercial offering and use of GBS (sometimes known as SBG) technology across all species and geographies from KeyGene.

Advantages

  • No prior knowledge of the genome sequence required
    Discover new SNPs in your species without the need for a reference genome.
  • Cost effective - Find thousands of markers in any sample number
     From a handful of samples for a few million reads to thousands of samples multiplexed on high-throughput NGS platforms.
  • Professional service and rapid turnaround times
    Licensed technology for all species, unique service provider for vegetable crops.
  • Compatible with high-throughput KASP genotyping
    Easy conversion of identified markers to LGC’s flexible and high-throughput KASP assays.
  • Bioinformatics support for any species
    Including sequence pre-processing, alignment and variant analysis.
Request a GBS consultation now

Applications

Applications for genotyping by sequencing include:

  • Co-dominant SNP Genotyping
  • Genetic Diversity Analysis
  • Genetic Mapping
  • QTL Mapping
  • Bulk Segregant Analysis
  • Mapping to Whole Genome Sequence
  • Discovery of (rare) SNP variants
  • Genome Wide Association Mapping
  • Genomic Selection/Prediction

For bespoke technical advice on GBS and sequencing based technologies from LGC please contact ngs@lgcgroup.com

Keygene N.V. owns patents and patent applications protecting its sequence based genotyping technologies, as described by Truong et al., PLoS ONE 7: e37565, 2012 . LGC has a licence from KeyGene N.V. to offer the GBS service for any organisms provided that use and application in human and animal shall be limited to research use only.