How do I determine the volume of buffer to add to my probe to prepare a stock concentration?
We recommend preparing a 100 µM stock solution of your probe. To prepare a 100 µM stock of reconstituted probe: Multiply the total nmol value found on the Certificate of Analysis, by 10. The resulting number will be the volume of diluent (in microliters) to add to your probe. Once resuspended in the appropriate volume of dilution buffer, the probe will be a 100 µM stock solution.
Tip: Be sure to make aliquots of the working concentration of probe to prevent freeze thaw cycles that may degrade the probe. We recommend freezing the stocks and aliquots either at -20 °C or -80 °C. Probes and primers can be stored in this state for over one year.