What buffer should I resuspend my oligonucleotide in?
For oligonucleotide suspension, we recommend preparing stock and working solutions using a TE buffer (10 mM Tris, 1 mM EDTA, pH 8.0) made with nuclease-free water. The EDTA serves to protect against microbial contamination. If your experiment cannot tolerate EDTA, you may use 10 mM Tris-Cl buffer. Suspension in water alone should be limited to nuclease-free water at physiological pHs, but is not recommended. Acidic conditions can lead to degradation of the oligonucleotide through depurination.
Notes: Fluorophores are sensitive to photobleaching. To minimize their exposure to light, we recommend using amber microtubes, wrapping the tube in foil, or else placing clear tubes into a box which is impermeable to light. Avoid freeze/thaw cycles by making working solutions and storing them in aliquots at -15 °C or cooler.