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ValuMix Assay for Gene Expression & qPCR


ValuMix AssayBiosearch Technologies can now deliver custom probes and primers combined together within a single tube according to the ratio you specify. This formulation minimizes pipetting error and simplifies your protocol by providing mixed oligonucleotides ready for amplification.

Primer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. The 0.5 nmol probe option is only available for mixed assays containing a FAM-BHQ® probe. Please refer to the Product Listings tab to view all dye-quencher combinations available for mixed sets comprising a custom BHQ probe and primers.

Best DEAL:
$79 – 0.5 nmol of FAM-BHQ probe mixed with primers

Instrument CALIBRATION:
FAM, CAL Fluor, and Quasar calibration dyes are available for online ordering.

Learn MORE:
Applying dual-labeled probes in multiplexed qPCR and gene expression analysis

NOTE:
Building Probes >30 Bases
Probe sequences longer than 30 bases are unlikely to have efficient quenching when the quencher is placed at the 3’ end. For such situations, please consider positioning the quencher internally instead.

Building Probes with an Internal Quencher (Currently available only with FAM)
Adding an internal quencher annotated with a T(...) indicates that the quencher is tethered to a Thymidine base. An additional T base will be introduced into the sequence when incorporating this internal modification.

Building probes with Degenerate Bases
(
What are Wobbles?)
To ensure the most efficient processing of your oligo, use
IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses or brackets, e.g. R or V instead of (A/G) or [A/G/C], etc.

See also:
BHQ® Probes | BHQplus® Probes | SNP Assays | Molecular Beacons | Scorpions® Primers | Dye Calibration Standards | Dyes from Biosearch Technologies



Product Listings

Catalog # Item Name Price Note
DLOM-RFB-5 FAM Dual-labeled BHQ-1 Probe with Primers, Mixed $119.00 5 nmol
DLOM-RFB-20 FAM Dual-labeled BHQ-1 Probe with Primers, Mixed $229.00 20 nmol
DLOM-RFB-05 FAM Dual-labeled BHQ-1 Probe with Primers, Mixed $79.00 0.5 nmol
DLOM-F(T-B1)-5 FAM Dual-labeled Internal T-BHQ-1 Probe with Primers, Mixed $209.00 5 nmol
DLOM-F(T-B1)-20 FAM Dual-labeled Internal T-BHQ-1 Probe with Primers, Mixed $379.00 20 nmol
DLOM-COB1-5 CAL Fluor Orange 560 Dual-labeled BHQ-1 Probe with Primers, Mixed $149.00 5 nmol
DLOM-COB1-20 CAL Fluor Orange 560 Dual-labeled BHQ-1 Probe with Primers, Mixed $279.00 20 nmol
DLOM-CAB2-5 CAL Fluor Red 610 Dual-labeled BHQ-2 Probe with Primers, Mixed $149.00 5 nmol
DLOM-CAB2-20 CAL Fluor Red 610 Dual-labeled BHQ-2 Probe with Primers, Mixed $279.00 20 nmol
DLOM-Q6B2-5 Quasar 670 Dual-labeled BHQ-2 Probe with Primers, Mixed $149.00 5 nmol
DLOM-Q6B2-20 Quasar 670 Dual-labeled BHQ-2 Probe with Primers, Mixed $279.00 20 nmol

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Probe

RP HPLC
 
 

Forward Primer
Reverse Primer

Enter your desired ratio at any tenth decimal between 1 and 4.5, or click on the vial.


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Probe:

All primers are Salt-Free
All primers are Salt-Free

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