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ValuMix gene expression assays

Product info

ValuMix for gene expression and qPCR delivers one BHQ Probe and two primers in a single tube. Valumix primer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options.

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Oligo set {{$index + 1}}

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ValuProbe
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This sequence is too short. Click here for help.
This sequence is too short. See Technology Overview
This sequence is shorter than recommended. See Technology Overview
This probe sequence is shorter than our recommended criteria for probe length. You may want to consider ordering this sequence as a dual-labeled BHQ probe or BHQplus® probe or MGB probe dual-labeled BHQ probe or BHQplus® probe or MGB probe instead.
You may want to consider ordering an internally quenched probe for sequences 30 bases or longer.
A surcharge has been applied to the price of this probe because the sequence length is longer than 35 bases.
A surcharge has been applied to the price of this probe because the sequence length is longer than 50 bases.
This oligo sequence is too long. Please contact customer service info@biosearchtech.com for a quote or click here for help.
This probe sequence is too long. Consider ordering this sequence as a BHQnova® probe. For additional assistance, click here for help.
Are you trying to order a Molecular Beacon? Click here to continue Click here to continue
If you are ordering an LNA please use an excel order form and email to info@biosearchtech.com
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
The internal modification you used in the sequence is not available with this 5' Modification. You need to remove it from your sequence.
Are you trying to include RNA bases? RNA bases are not allowed in standard BHQ probes and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in BHQnova probes and so the R character will be treated as a DNA wobble instead (A/G). Please click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in standard BHQplus probes and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in standard MGB probes and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
RNA bases are not allowed in standard Molecular Beacons. Please use the custom oligo form custom oligo form to order these modifications or click here for help.
Inosine modifications are not allowed in standard BHQ probes. Please order on the custom oligo form custom oligo form or click here for help.
Inosine modifications are not allowed in standard Nova probes. Please order on the custom oligo form custom oligo form or click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
An invalid character has been entered into the sequence. For additional assistance, click here for help.
Illegal character(s) were entered. Please review your sequence information.
Internal modifications are not allowed in standard Molecular Beacons. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
Internal modifications are not allowed in standard BHQplus probes. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
Internal modifications are not allowed in standard MGB probes. For additional assistance, click here for help.
Internal modifications are not allowed in standard Nova probes. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
The sequence contains open brackets. Please, verify your sequence.
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Probe nmol options
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  • {{item.minNmol}} nmol

Purification
Item price TBD
Field required.
This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Inosine modifications must be included in brackets such as [I] or [i].
Internal modifications are not allowed in standard primers. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
No special modifications are allowed in BHQplus® probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
No special modifications are allowed in MGB probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
Invalid character(s) were entered. Please review your sequence information.
Item price
Field required.
This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Inosine modifications must be included in brackets such as [I] or [i].
Internal modifications are not allowed in standard primers. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
No special modifications are allowed in BHQplus® probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
No special modifications are allowed in MGB probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
Invalid character(s) were entered. Please review your sequence information.
Item price
nmol options
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  • {{primerScale.purifications[order.purificationIndex()].minNmol}} nmol
Clear scale

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Probe catalog #: {{order.probe1.fivePrime.threePrime.item.catalogId}} Primer catalog #: {{order.primer.item.catalogId}} Assay subtotal: {{(order.probe1.fivePrime.threePrime.item.price + order.probe1.fivePrime.threePrime.item.surcharge + order.primer.primer2.price + order.primer.primer1.price)*order.quantity | currency}} Qty another assay
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Assay #{{$index + 1}}

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You need to change the assay name because it has already been used.
Probe name
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This sequence is too short. Click here for help.
This sequence is too short. See Technology Overview.
This sequence is shorter than recommended. See Technology Overview.
This probe sequence is shorter than our recommended criteria for probe length. You may want to consider ordering this sequence as a MGB probe or MGB probe instead.
If you are ordering an LNA please use an excel order form and email to info@biosearchtech.com
You may want to consider ordering an internally quenched probe for sequences 30 bases or longer.
This probe sequence is too long. Consider ordering this sequence as a BHQnova® probe. For additional assistance, click here for help.
This probe sequence is too long. ValuMix assays only allow a probe length up to 35 bases. Please order this probe as a Dual-Labeled BHQ® Probe or click here for help.
Are you trying to order a Molecular Beacon? Click here to continue Click here to continue
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
The internal modification you used in the sequence is not available with this 5' Modification. You need to remove it from your sequence.
Internal modifications are not allowed in standard MGB probes. For additional assistance, click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in ValuMix assays and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
This modification is not allowed in ValuMix assays. Please use the custom oligo form custom oligo form or click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
An invalid character has been entered into the sequence. For additional assistance, click here for help.
This modification is not allowed for ValuMix assays. Please use the custom oligo order form custom oligo order form or click here for help.
The sequence contains open brackets. Please, verify your sequence.
Field required.
add
Probe nmol options
Field required.
  • {{item.minNmol}} nmol

Purification
Item price TBD

Forward primer

Sequence name
Purification All primers are salt-free
Field required.
This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Primers for this type of ValuMix Assay cannot include internal modifications. Click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
This modification is not allowed in ValuMix assays. Please use the custom oligo form custom oligo form or click here for help.

Reverse primer

Sequence name
Purification All primers are salt-free
Field required.
This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Primers for this type of ValuMix Assay cannot include internal modifications. Click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
This modification is not allowed in ValuMix assays. Please use the custom oligo form custom oligo form or click here for help.

1:


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You are ordering:
Probe catalog #: {{order.probe1.fivePrime.threePrime.item.catalogId}} Assay subtotal: {{(order.probe1.fivePrime.threePrime.item.price + order.primer.primer2.price + order.primer.primer1.price)*order.quantity | currency}} Qty another assay
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Product listing

Catalog # Item name Price Note
{{listedItem.CatalogId}} {{listedItem.CatalogId}} 		{{listedItem.Price|currency}} TBD

Technical specs

BHQ ValuMix for qPCR and gene expression specifications
PROBE LENGTH 17-30 bases recommended
DYES AVAILABLE FAM, TET, CAL Fluor Gold 540, CIV-550, JOE, HEX, CAL Fluor Orange 560, CAL Fluor Red 610, Quasar 705
QUENCHER AVAILABLE BHQ-1, BHQ-2
YIELD

0.5 nmol probe/0.5-2.5 nmol primer (FAM)
5 nmol probe/5-22 nmol primer
20 nmol probe/20-90 nmol primer
PURIFICATION Probe - RP HPLC
Primer - salt-free
DELIVERY FORMAT Dry or In Solution
QUALITY CONTROL MS and UHPLC
QUALITY STANDARD ISO 9001
SHELF LIFE 12 months from date of manufacture
SHIPPING CONDITIONS Ambient temperature or dry ice (in solution)

Absorption and emission spectral charts

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