Product info

BHQ™ Probes are traditional, linear, dual-labelled FRET probes, typically 20 to 30 bases in length, with a fluorophore and quencher covalently attached to the 5' and 3' ends, respectively. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridisation to its complementary sequence. BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences.

Key benefits:

  • Unmatched quality and technical support from the original makers of the BHQ quenchers
  • Complete suite of fluorescent dyes spanning the entire visible spectrum
  • Simple probe design and implementation with consistent and reliable performance
 

Design versatility without performance variability

When it comes to assay design, we don’t believe in a one-dye-fits-all strategy. Our wide selection of dyes empowers you to design the best PCR or qPCR probe for your application without sacrificing performance. We have paired each dye with the BHQ quencher that offers optimal quenching efficiency. With our versatile BHQ Probes, you can design with confidence.

5' fluorescent dye

Abs (nm)

Em (nm)

3' quencher

  FAM 495 520 BHQ-1
  TET 521 536 BHQ-1
  CAL Fluor Gold 540 522 544 BHQ-1
  CIV-550 530 550 BHQ-1*, BHQ-2
  JOE 529 555 BHQ-1
  HEX 535 556 BHQ-1
  CAL Fluor Orange 560 538 559 BHQ-1
  Quasar 570 548 566 BHQ-2
  Cy3 549 566 BHQ-2
  TAMRA 557 583 BHQ-2
  CAL Fluor Red 590 569 591 BHQ-2
  ROX 586 610 BHQ-2
  CAL Fluor Red 610 590 610 BHQ-2
  CAL Fluor Red 635 618 637 BHQ-2
  Cy5 646 669 BHQ-2
  Quasar 670 647 670 BHQ-2*, BHQ-3
  Quasar 705 690 705 BHQ-2*, BHQ-3

* Recommended quencher

Use the spectral overlay tool to view compatible dyes for your instrument. Learn more

High quality FAM/BHQ-1 ValuProbes at a low price

Our ValuProbe is comprised of 10 nmols probe with a 5' FAM and a 3' BHQ-1 quencher, delivered dried down, purified via Reverse Phase HPLC, and verified via mass spectrometry and UHPLC.

How to order a ValuProbe

  • Navigate to the Order now tab at the top of this page.
  • Select   valuprobe   to populate ValuProbe parameters.
  • Enter your probe name and sequence.
  • Add primers (optional).
  • Add to basket.

A ValuMix assay built exactly to your specifications

BHQ Probes in the ValuMix assay format for qPCR and gene expression include a BHQ Probe and two primers mixed at your designated primer to probe ratio between 1 and 4.5. Simplify and streamline your experimental setup while reducing any chance of pipetting error.

Key features of ValuMix assays

  • Probes purified via RP HPLC
  • Salt-free primers
  • Wide selection of dyes: FAM, TET, CAL Fluor Gold 540, CIV-550, HEX, CAL Fluor Orange 560, CAL Fluor Red 610 and Quasar 670
  • 0.5 nmol (FAM only), 5 nmol, or 20 nmol delivered

Design considerations for BHQ Probe

  • Target a probe length typically between 20 and 30 bases.
  • If your sequence is shorter than 17 bases, consider ordering this sequence as a BHQplus Probe.
  • BHQ Probe sequences longer than 30 bases are unlikely to have efficient quenching. Consider ordering a BHQnova for sequences 30 bases or longer. For assistance, please contact us.
  • Target a %GC content between 30% and 80%.
  • Avoid runs of identical nucleotides.
  • Do not place a guanosine on the 5′ end. A guanosine next to the reporter dye will alter the fluorescence.
  • Avoid guanosine on the 3′ end (ex. 5′-...GGG-3′ or 5′-...GGAG-3′).
  • Avoid 4 consecutive guanosines as these can form a stable secondary structure.
  • For FAM-labelled probes, avoid a guanosine in the second position on the 5′ end.
  • Building probes with an internal quencher (currently available with FAM only) introduces an additional T base into the sequence.
  • Use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc.

Order now

BHQ ValuMix assays for qPCR and gene expression offer the convenience of having a custom, dual-labeled BHQ probe and primer pair delivered in exact amounts within a single tube at your selected probe-to-primer ratio (between 1:1 to 1:4.5).

 

Order BHQ probes and primers delivered each in separate tubes

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You may want to consider ordering an internally quenched probe for sequences 30 bases or longer.
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Are you trying to include RNA bases? RNA bases are not allowed in standard BHQ probes and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in BHQnova probes and so the R character will be treated as a DNA wobble instead (A/G). Please click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in standard BHQplus probes and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in standard MGB probes and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
RNA bases are not allowed in standard Molecular Beacons. Please use the custom oligo form custom oligo form to order these modifications or click here for help.
Inosine modifications are not allowed in standard BHQ probes. Please order on the custom oligo form custom oligo form or click here for help.
Inosine modifications are not allowed in standard Nova probes. Please order on the custom oligo form custom oligo form or click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
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Internal modifications are not allowed in standard Molecular Beacons. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
Internal modifications are not allowed in standard BHQplus probes. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
Internal modifications are not allowed in standard MGB probes. For additional assistance, click here for help.
Internal modifications are not allowed in standard Nova probes. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
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Inosine modifications must be included in brackets such as [I] or [i].
Internal modifications are not allowed in standard primers. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
No special modifications are allowed in BHQplus® probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
No special modifications are allowed in MGB probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
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This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Inosine modifications must be included in brackets such as [I] or [i].
Internal modifications are not allowed in standard primers. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
No special modifications are allowed in BHQplus® probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
No special modifications are allowed in MGB probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
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Technical Specs

BHQ Probe specifications
Probe length 17-30 bases
Dyes FAM, TET, CAL Fluor gold 540, CIV-550, JOE, HEX, CAL Fluor Orange 560, Quasar 570, TAMRA, CAL Fluor Red 590, ROX, CAL Fluor Red 610, CAL Fluor Red 635, Quasar 670, Quasar 705
Quenchers BHQ-1, BHQ-2, BHQ-3, TAMRA
Yield Dye dependent (see product listing)
Purification DUAL HPLC
ValuProbe purified via RP HPLC
Delivery format Dry or in solution
Quality control MS and UHPLC
Quality standard ISO 9001 or ISO 13485
Shelf life 12 months from date of manufacture

 

BHQ ValuMix for qPCR and gene expression
Probe length 17 - 30 bases recommended
Dyes FAM, TET, CAL Fluor gold 540, CIV-550, JOE, HEX, CAL Fluor Orange 560, Quasar 670
Quenchers BHQ-1, BHQ-2
Yield 0.5 nmol probe/0.5 - 2.5 nmol primer (FAM)
5 nmol probe/5 - 22 nmol primer
20 nmol probe/20 - 90 nmol primer
Purification Probe - RP HPLC
Primer - salt-free
Delivery format Dry or in solution
Quality control MS and UHPLC
Quality standard ISO 9001
Shelf life 12 months from date of manufacture
Shipping Ambient for dry format
Dry ice if in solution

 

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