RNA FISH for cancer targets

Visualization and quantification of single RNA transcripts, using Stellaris® RNA fluorescence in situ hybridization, offers the unique ability to observe altered disease-specific expression in fixed cells or intact tissue

Stellaris RNA FISH, offers the unique ability to observe altered disease-specific expression in fixed cells or intact tissue. Gene expression profiles are complex and can vary immensely between regions of tissue, cells, and even regions of a single nucleus, and therefore require advanced methods of analysis.

Stellaris Multiplexing

Simultaneous RNA FISH and Immunofluorescence

Image of simultaneous Stellaris RNA FISH and immunofluorescence assay. HER2 mRNA molecules (green), protein marker HER2 (pink), DAPI (red) in human SK-BR-3 cells (AV Orjalo Biosearch Tech.)

Publication Highlights

Over 33% of publications citing Stellaris RNA FISH publish in Cell, Nature, or Science

Visit our Citation Center for the complete list.

Stellaris in Tissue

Post-transcriptional Modifications Contribute to the Upregulation of Cyclin D2 in Multiple Myeloma.
Misiewicz-Krzeminska et al. Clinical Cancer Research. 2016. url: https://dx.doi.org/10.1158/1078-0432.CCR-14-2796

G1/S Inhibitors and the SWI/SNF Complex Control Cell-Cycle Exit during Muscle Differentiation.
Ruijtenberg et al. Cell. 2015. url: https://dx.doi.org/10.1016/j.cell.2015.06.013

Genome-wide profiling of p53-regulated enhancer RNAs uncovers a subset of enhancers controlled by a lncRNA.
Leveille et al. Nature Communications. 2015. url: https://dx.doi.org/10.1038/ncomms7520

Niche appropriation by Drosophila intestinal stem cell tumours.
Patel et al. Nature Cell Biology. 2015. url: https://dx.doi.org/10.1038/ncb3214

FuseFISH: robust detection of transcribed gene fusions in single cells.
Semrau et al. Cell reports. 2014. url: https://dx.doi.org/10.1016/j.celrep.2013.12.002

Proteostatic Control of Telomerase Function through TRiC-Mediated Folding of TCAB1.
Freund et al. Cell. 2014. url: https://dx.doi.org/10.1016/j.cell.2014.10.059

Single-molecule transcript counting of stem-cell markers in the mouse intesting.
Itzkovitz et al. Nature Cell Biology. 2011. url: https://dx.doi.org/10.1038/ncb2384

The Stellaris RNA FISH Method

The Stellaris Method