Stellaris RNA FISH: Detection, Localization, and Quantification of RNA at the (Sub-)Cellular Level
Stellaris® RNA FISH (fluorescence in situ hybridization) is a RNA visualization method that allows simultaneous detection, localization, and quantification of individual RNA molecules at the sub-cellular level in fixed samples using widefield or confocal fluorescence microscopy. A set of Stellaris RNA FISH Probes comprises multiple oligonucleotides with different sequences each with a fluorescent label that collectively bind along the same target transcript to produce a punctate signal.
The Stellaris RNA FISH technology follows a simple protocol, does not use exotic reagents, is inexpensive and platform-independent. It also offers same-day results, and is versatile both in terms of sample type and application. Stellaris RNA FISH probes may even be labeled with several different dyes, to enable simultaneous multiplex detection of different RNA targets. Finally, scientists can address the stochastic nature of gene expression and visualize RNA through direct detection without isolation, purification, and amplification.