DNA probes

We provide probe and primer formats, including dual-labeled BHQ® probes, BHQplus® probes, Molecular Beacons, and Scorpions® primers.

Dual-Labeled BHQ® Probes

View the selection of fluorophore-quencher combinations for dual-labeled probes available from LGC Biosearch. We synthesize high quality dual-labeled probes followed by HPLC purification to ensure every order meets purity requirements. Every oligo receives rigorous QC by electrospray mass spectroscopy before releasing for shipment.

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BHQplus Probes

An advanced technology for qPCR and SNP genotyping, the BHQplus® probe is a compact oligo fortified with our signature duplex stabilizing chemistry to elevate the melting temperature and enhance target specificity. BHQplus probes are the optimal choice for marker-assisted selection in the agricultural industry, and to research the polymorphisms that may influence human health.

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Scorpions Primers

Scorpions® primers combine the probe and primer into one oligo to accomplish a uni-molecular signaling mechanism. Enzymatic hydrolysis of the probe during PCR cycling is not required for signal release. View LGC Biosearch’s selection of Scorpions primers.

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Molecular Beacons

Molecular Beacons have short complementary sequences that fold into a stem-loop structure. This hairpin conformation positions the fluorophore and quencher very close together in space, for remarkably efficient quenching. LGC Biosearch currently offers Molecular Beacons labeled with either a Black Hole Quencher® or a DABCYL dye. These quenchers are paired with a variety of fluorescent reporters.

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BHQnova Probes

The most difficult qPCR applications demand double-quenched probes for optimum performance. BHQnova™ probes have improved quenching efficiency compared to traditional end-labeled probes, while also improving signal release upon amplification. BHQnova is most advantageous in longer probe designs, typically those over 25 bases, to boost the signal-to-noise ratio by overcoming the upper limit on sequence length.

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