Product info

Spend less time at the bench and more time on your research.

ValuMix™ assays for qPCR and gene expression offer the convenience of having a custom, dual-labeled probe and two primers delivered in exact amounts within a single tube at your selected probe-to-primer ratio.

Use this technology if you need one custom dual-labled probe and two primers. If you require two probes and two primers, please use the ValuMix assay for SNP genotyping

Choose from four probe chemistries: BHQ™, BHQplus™, MGB or LNA Probes. Each probe type is available in three standard sizes and is compatible with a complete set of dye options to ensure optimal performance with your instrument’s optics.

Key benefits:

  • Flexible design: With four options, it’s easy to pilot different probe chemistries in your assay design and development to ensure optimal targeting and assay performance.
  • Versatile: Select the best dye for your instrument’s optics. Choose from FAM, TET, CAL Fluor Gold 540, CIV-550, HEX, CAL Fluor Orange 560, CAL Fluor Red 610 and Quasar 670.
  • Adjustable probe-to-primer ratio: Take the worry out of oligo dilution calculations with oligos provided at the exact quantities you specify.
  • Less time spent at the bench: Simplify qPCR setup by reducing pipetting steps for rapid reaction setup times.
 

 

5' fluorescent dye

Abs (nm)

Em (nm)

3' quencher

  FAM 495 520 BHQ-1 or EDQ*
  TET 521 536 BHQ-1 or EDQ
  CAL Fluor Gold 540 522 544 BHQ-1 or EDQ
  CIV-550 530 550 BHQ-1 or EDQ
  HEX 535 556 BHQ-1 or EDQ
  CAL Fluor Orange 560 538 559 BHQ-1 or EDQ
  CAL Fluor Red 610 590 610 BHQ-2
  Quasar 670 647 670 BHQ-2

 

* MGB Probes come with Eclipse Dark Quencher (EDQ). BHQ, BHQplus and LNA Probes are available with BHQ quenchers.

Use the spectral overlay tool to view compatible dyes for your instrument. Learn more

 

Adjust primer concentration to suit your assay conditions

You can select a probe-to-primer ratio anywhere from 1:1 to 1:4.5 to deliver the exact assay conditions you require. The adjustable ratio makes it easy for you to integrate ValuMix assays into your existing qPCR protocols.

Choose from one of three duplex stabilising probe chemistries

Duplex stabilising chemistries enable shorter probe design while maintaining the optimal melting temperature (Tm). Shorter probes provide improved signal-to-noise ratio and stabilising chemistries enhance detection of difficult targets, such as AT-rich sequences.

LGC Biosearch Technologies is the only supplier that offers three duplex stabilising chemistries (BHQplus, MGB, and LNA Probes), which gives you flexibility to optimise your assay and maximise performance.

  • BHQplus Probes: BHQplus hydrolysis probes consist of a 5′ fluorescent reporter dye, a 3′ BHQ, and modified C and T nucleotides throughout the probe sequence which are responsible for enhanced probe-target binding stability. The compact sequences of BHQplus Probes are simple to design and typically 15-25 bases in length.
     
  • Minor Groove Binder (MGB) Probes: MGB Probes consist of a 5′ fluorescent reporter dye and a 3′ Eclipse Dark Quencher (EDQ) conjugated to a MGB moiety that stabilises the probe-target duplex. The simple and short 8-30 base MGB Probe design enables improved sequence specificity and assay sensitivity.
     
  • Locked Nucleic Acid (LNA) Probes: LNA hydrolysis probes consist of a 5′ fluorescent reporter dye, a 3′ BHQ, and up to seven LNA bases in the probe sequence. LNAs are modified RNA bases in which the ribose is “locked” with a methylene bridge, fixing it in the C3'-endo conformation. LNA bases have increased affinity for complementary DNA bases, which increases the probe melting temperature and enables a shorter probe design. Each LNA monomer substituted into the 15-25 base probe sequence increases the Tm of the duplex by 2-8 °C allowing for an exact fine-tuning. Order LNA Probes for qPCR and gene expression.

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Oligo set {{$index + 1}}

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This sequence is too short. See Technology Overview
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This probe sequence is shorter than our recommended criteria for probe length. You may want to consider ordering this sequence as a dual-labeled BHQ probe or BHQplus® probe or MGB probe dual-labeled BHQ probe or BHQplus® probe or MGB probe instead.
You may want to consider ordering an internally quenched probe for sequences 30 bases or longer.
A surcharge has been applied to the price of this probe because the sequence length is longer than 35 bases.
A surcharge has been applied to the price of this probe because the sequence length is longer than 50 bases.
This oligo sequence is too long. Please contact customer service info@biosearchtech.com for a quote or click here for help.
This probe sequence is too long. Consider ordering this sequence as a BHQnova® probe. For additional assistance, click here for help.
Are you trying to order a Molecular Beacon? Click here to continue Click here to continue
If you are ordering an LNA please use an excel order form and email to info@biosearchtech.com
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
The internal modification you used in the sequence is not available with this 5' Modification. You need to remove it from your sequence.
Are you trying to include RNA bases? RNA bases are not allowed in standard BHQ probes and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in BHQnova probes and so the R character will be treated as a DNA wobble instead (A/G). Please click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in standard BHQplus probes and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in standard MGB probes and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
RNA bases are not allowed in standard Molecular Beacons. Please use the custom oligo form custom oligo form to order these modifications or click here for help.
Inosine modifications are not allowed in standard BHQ probes. Please order on the custom oligo form custom oligo form or click here for help.
Inosine modifications are not allowed in standard Nova probes. Please order on the custom oligo form custom oligo form or click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
An invalid character has been entered into the sequence. For additional assistance, click here for help.
Illegal character(s) were entered. Please review your sequence information.
Internal modifications are not allowed in standard Molecular Beacons. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
Internal modifications are not allowed in standard BHQplus probes. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
Internal modifications are not allowed in standard MGB probes. For additional assistance, click here for help.
Internal modifications are not allowed in standard Nova probes. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
The sequence contains open brackets. Please, verify your sequence.
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Probe nmol options
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Purification
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This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Inosine modifications must be included in brackets such as [I] or [i].
Internal modifications are not allowed in standard primers. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
No special modifications are allowed in BHQplus® probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
No special modifications are allowed in MGB probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
Invalid character(s) were entered. Please review your sequence information.
Item price
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This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Inosine modifications must be included in brackets such as [I] or [i].
Internal modifications are not allowed in standard primers. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
No special modifications are allowed in BHQplus® probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
No special modifications are allowed in MGB probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
Invalid character(s) were entered. Please review your sequence information.
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nmol options
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Assay #{{$index + 1}}

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Probe name
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This sequence is too short. Click here for help.
This sequence is too short. See Technology Overview.
This sequence is shorter than recommended. See Technology Overview.
This probe sequence is shorter than our recommended criteria for probe length. You may want to consider ordering this sequence as a MGB probe or MGB probe instead.
If you are ordering an LNA please use an excel order form and email to info@biosearchtech.com
You may want to consider ordering an internally quenched probe for sequences 30 bases or longer.
This probe sequence is too long. Consider ordering this sequence as a BHQnova® probe. For additional assistance, click here for help.
This probe sequence is too long. ValuMix assays only allow a probe length up to 35 bases. Please order this probe as a Dual-Labeled BHQ® Probe or click here for help.
Are you trying to order a Molecular Beacon? Click here to continue Click here to continue
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
The internal modification you used in the sequence is not available with this 5' Modification. You need to remove it from your sequence.
Internal modifications are not allowed in standard MGB probes. For additional assistance, click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in ValuMix assays and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
This modification is not allowed in ValuMix assays. Please use the custom oligo form custom oligo form or click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
An invalid character has been entered into the sequence. For additional assistance, click here for help.
This modification is not allowed for ValuMix assays. Please use the custom oligo order form custom oligo order form or click here for help.
The sequence contains open brackets. Please, verify your sequence.
Field required.
add
Probe nmol options
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  • {{item.minNmol}} nmol

Purification
Item price TBD

Forward primer

Sequence name
Purification All primers are salt-free
Field required.
This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Primers for this type of ValuMix Assay cannot include internal modifications. Click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
This modification is not allowed in ValuMix assays. Please use the custom oligo form custom oligo form or click here for help.

Reverse primer

Sequence name
Purification All primers are salt-free
Field required.
This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Primers for this type of ValuMix Assay cannot include internal modifications. Click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
This modification is not allowed in ValuMix assays. Please use the custom oligo form custom oligo form or click here for help.

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Probe catalog #: {{order.probe1.fivePrime.threePrime.item.catalogId}} Assay subtotal: {{(order.probe1.fivePrime.threePrime.item.price + order.primer.primer2.price + order.primer.primer1.price)*order.quantity | currency}} Qty another assay
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Product listing

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Technical specs

BHQ ValuMix for qPCR and gene expression
Probe length 17 - 30 bases
Dyes FAM, TET, CAL Fluor Gold 540, CIV-550, HEX, CAL Fluor Orange 560, CAL Fluor Red 610, Quasar 670
Quenchers BHQ-1, BHQ-2
Yield 0.5 nmol probe / 0.5-2.5 nmol primer (FAM)
5 nmol probe / 5-22 nmol primer
20 nmol probe / 20-90 nmol primer
Purification Probe - RP HPLC
Primer - salt-free
Delivery format Dry or In Solution
Quality control MS and UHPLC
Quality standard ISO 9001
Shelf life 12 months from date of manufacture
Shipping Ambient temperature or dry ice (in solution)

 

BHQplus ValuMix for qPCR and gene expression
Probe length 15 - 25 bases
Dyes FAM, TET, CAL Fluor Gold 540, CIV-550, HEX, CAL Fluor Orange 560, CAL Fluor Red 610, Quasar 670
Quenchers BHQ-1, BHQ-2
Yield 0.5 nmol probe / 0.5-2.5 nmol primer (FAM)
5 nmol probe / 5-22.5 nmol primer
20 nmol probe / 20-90 nmol primer
Purification Probe - RP HPLC
Primer - salt-free
Delivery format Dry or In Solution
Quality control MS and UHPLC
Quality standard ISO 9001
Shelf life 12 months from date of manufacture
Shipping Ambient temperature or dry ice (in solution)

 

MGB ValuMix for qPCR and gene expression
Probe length 8 - 30 bases
Dyes FAM, TET, CAL Fluor Gold 540, CIV-550, HEX, CAL Fluor Orange 560
Quenchers Eclipse Dark Quencher (EDQ)
Yield 0.5 nmol probe / 0.5-2.5 nmol primer (FAM)
5 nmol probe / 5-22.5 nmol primer
20 nmol probe / 20-90 nmol primer
Purification Probe - RP HPLC
Primer - salt-free
Delivery format Dry or in solution (TrisHCl, T10E0.1, T10E1)
Quality control MS and UHPLC
Quality standard ISO 9001
Shelf life 12 months from date of manufacture
Shipping Ambient temperature or dry ice (in solution)

 

LNA ValuMix for qPCR and gene expression
LNA bases Up to 7 LNA bases in probe sequence
Dyes FAM, TET, CAL Fluor Gold 540, CIV-550, HEX, CAL Fluor Orange 560, CAL Fluor Red 610, Quasar 670
Quenchers BHQ-1, BHQ-2
Yield 0.5 nmol probe / 0.5-2.5 nmol primer (FAM)
5 nmol probe / 5-22.5 nmol primer
20 nmol probe / 20-90 nmol primer
Purification Probe - RP HPLC
Primer - salt-free
Delivery format Dry or in solution (TrisHCl, T10E0.1, T10E1)
Quality control MS and UHPLC
Quality standard ISO 9001
Shelf life 12 months from date of manufacture
Shipping Ambient temperature or dry ice (in solution)

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