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Product Info

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Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences.

BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options.

Design Concerns for BHQ probes

Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact techsupport@biosearchtech.com with any questions regarding probe design.

NOTE:

  • Building Probes with an Internal Quencher (Currently available with FAM only)
  • Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification.
  • Building Probes with Wobble/Degenerate Bases (What are Wobbles?)
  • To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc.

 

Best DEAL:

  • $95 ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days

Instrument CALIBRATION:

Learn MORE:

Assay DESIGN:

  • Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

Product Listing

Catalog # Item Name Price Note
{{listedItem.CatalogId}} {{listedItem.CatalogId}}
{{listedItem.Price|currency}} TBD

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Oligo set {{$index + 1}}

{{error.message}}
You need to change the AssayName because it has already been used.
Field required.
ValuProbe
Field required.
Field required.
This sequence is too short. Click here for help.
This probe sequence is shorter than our recommended criteria for probe length. You may want to consider ordering this sequence as a dual-labeled BHQ probe or BHQplus® probe dual-labeled BHQ probe or BHQplus® probe instead.
You may want to consider ordering an internally quenched probe for sequences 30 bases or longer.
A surcharge has been applied to the price of this probe because the sequence length is longer than 35 bases.
A surcharge has been applied to the price of this probe because the sequence length is longer than 50 bases.
This oligo sequence is too long. Please contact customer service info@biosearchtech.com for a quote or click here for help.
Are you trying to order a Molecular Beacon? Click here to continue Click here to continue
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
The internal modification you used in the sequence is not available with this 5' Modification. You need to remove it from your sequence.
Are you trying to include RNA bases? RNA bases are not allowed in standard BHQ probes and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in BHQnova probes and so the R character will be treated as a DNA wobble instead (A/G). Please click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in standard BHQplus probes and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
RNA bases are not allowed in standard Molecular Beacons. Please use the custom oligo form custom oligo form to order these modifications or click here for help.
Inosine modifications are not allowed in standard BHQ probes. Please order on the custom oligo form custom oligo form or click here for help.
Inosine modifications are not allowed in standard Nova probes. Please order on the custom oligo form custom oligo form or click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
Illegal character(s) were entered. Please review your sequence information.
Internal modifications are not allowed in standard Molecular Beacons. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
Internal modifications are not allowed in standard BHQplus probes. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
Internal modifications are not allowed in standard Nova probes. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
The sequence contains open brackets. Please, verify your sequence.
Field required.
add
Probe Scale
Field required.

Purification
Item Price TBD
Field required.
This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Inosine modifications must be included in brackets such as [I] or [i].
Internal modifications are not allowed in standard primers. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
No special modifications are allowed in BHQplus® probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
Illegal character(s) were entered. Please review your sequence information.
Item Price
Field required.
This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Inosine modifications must be included in brackets such as [I] or [i].
Internal modifications are not allowed in standard primers. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
No special modifications are allowed in BHQplus® probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
Illegal character(s) were entered. Please review your sequence information.
Item Price
Primer Scale
Field required.
Clear Scale

Field required.
You Are Ordering:
Probe Catalog #: {{order.probe1.fivePrime.threePrime.item.catalogId}} Primer Catalog #: {{order.primer.item.catalogId}} Assay Subtotal: {{(order.probe1.fivePrime.threePrime.item.price + order.probe1.fivePrime.threePrime.item.surcharge + order.primer.primer2.price + order.primer.primer1.price)*order.quantity | currency}} QTY ANOTHER Assay
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Assay #{{$index + 1}}

Field required.
You need to change the AssayName because it has already been used.
Probe Name
Field required.
Field required.
This sequence is too short. Click here for help.
This probe sequence is shorter than our recommended criteria for probe length. You may want to consider ordering this sequence as a BHQplus® probe BHQplus® probe instead.
You may want to consider ordering an internally quenched probe for sequences 30 bases or longer.
This probe sequence is too long. ValuMix assays only allow a probe length up to 35 bases. Please order this probe as a Dual-Labeled BHQ® Probe or click here for help.
Are you trying to order a Molecular Beacon? Click here to continue Click here to continue
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
The internal modification you used in the sequence is not available with this 5' Modification. You need to remove it from your sequence.
Are you trying to include RNA bases? RNA bases are not allowed in ValuMix assays and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
This modification is not allowed in ValuMix assays. Please use the custom oligo form custom oligo form or click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
This modification is not allowed for ValuMix assays. Please use the custom oligo order form custom oligo order form or click here for help.
The sequence contains open brackets. Please, verify your sequence.
Field required.
add
Probe Scale
Field required.

Purification
Item Price TBD

Forward Primer

Sequence Name
Purification All primers are Salt-Free
Field required.
This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Primers for this type of ValuMix Assay cannot include internal modifications. Click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
This modification is not allowed in ValuMix assays. Please use the custom oligo form custom oligo form or click here for help.

Reverse Primer

Sequence Name
Purification All primers are Salt-Free
Field required.
This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Primers for this type of ValuMix Assay cannot include internal modifications. Click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
This modification is not allowed in ValuMix assays. Please use the custom oligo form custom oligo form or click here for help.

1:


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You Are Ordering:
Probe Catalog #: {{order.probe1.fivePrime.threePrime.item.catalogId}} Assay Subtotal: {{(order.probe1.fivePrime.threePrime.item.price + order.primer.primer2.price + order.primer.primer1.price)*order.quantity | currency}} QTY ANOTHER Assay
Max Assay forms reached, Please add current assays to your cart, then return to form to continue adding more assays
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Subtotal: {{ctrl.getTotalPrice() | currency}} add to cart add to cart