RNA FISH for stem cell research
Stem cells hold great promise for the treatment of various diseases due to their capacity for self-renewal and ability to differentiate. Stellaris™ RNA fluorescence in situ hybridisation offers a unique insight into the expression patterns of embryonic and adult stem cells.
Visualisation, quantification, and localization of individual RNA molecules provide insight into the unique expression patterns of embryonic and adult stem cells. Though stem cells hold great promise for the treatment of various diseases due to their capacity for self-renewal and ability to differentiate, a basic understanding of stemness remains elusive. Advanced methods of analysis are required for the complex gene expression present in stem cells.
Custom or DesignReady Probe sets
LGC, Biosearch Technologies‘ free, online Stellaris Probe designer allows you to craft Stellaris RNA FISH probes with optimal binding properties for your target RNA sequence. A custom Stellaris RNA FISH probe set is a blend of up to 48 oligos labelled with a fluorophore.
We also offer DesignReady probe sets which are professionally designed and go through rigorous bioinformatics analysis to ensure specificity. These made-to-order probe sets include stem cell-specific targets across a wide range of model organisms. More information and current protocols are available online.
Detect, localise, and quantify RNA molecules in cells or intact tissue
Biosearch Technologies’ Stellaris RNA FISH probes, can quantify and localise RNA within cells, tissue sections, or whole mount tissue where the morphology is preserved. A Stellaris FISH probe set comprises multiple oligonucleotides targeting a single RNA target. The fluorescently labeled probes bind along the target transcript, and combine to produce a punctate signal for an individual RNA molecule.
Image of Stellaris RNA FISH with Immunofluorescence. XIST (red), GFAP protein (green). Photo credit: N. Khoury
Over 33% of publications citing Stellaris RNA FISH publish in Cell, Nature, or Science
Visit our Citation Center for the complete list.