qPCR applications, calibration and troubleshooting

How do I adjust my thermal cycler’s settings to account for the BHQ quencher?

How do I calibrate my instrument for the CAL Fluor and Quasar Dyes?

Which dyes are compatible with my thermal cycler?

I am having a problem with background drift with my BHQ™ Probe. What might the cause be?

I am not getting any signal generation with my BHQ™ Probe. What might cause this?

I am performing real time qPCR and my negative controls are amplifying. Why would this happen?

Why is my BHQ-Pulsar 650 probe not detected in a singleplex reaction on my LightCycler?

Why do I get different Ct values for the same probe sequence when labelled with a different dye?

How many PCR reactions will I be able to run with my probe or primer?

I am a beginner at real-time qPCR. Does Biosearch Technologies have information which will help me to design my assay?

Does Biosearch Technologies have software available for designing probes and primers?

Is there a formula for calculating the efficiency of a qPCR reaction?

Does Biosearch Technologies make available information on multiplex qPCR?

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