The I-TAMB modification incorporates a fluorescent tetramethylrhodamine (TAMRA) dye internally or at the 5' terminus of an oligonucleotide. TAMRA modified oligonucleotides can be used in a wide array of applications, including dual-labeled fluorogenic probes for real-time PCR. TAMRA is a rhodamine dye and this product is prepared using the 6-carboxy isomer. The fluorophore is conjugated via a ten atom linker to a modified thymidine which can participate in hybridization. TAMRA fluoresces in yellow-orange region of the visible spectrum and can be effectively quenched by BHQ®-2 dye.
Properties:
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Formula:
C43H47N6O13P
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Molecular Weight:
886.84
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Absorption Maximum (Lambda Max):
557
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Extinction Coefficient at Lambda max:
90000
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Extinction Coefficient at 260 nm:
40380
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Fluorescence Maximum:
583
Product usage:
-
The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
870.84
