Technical specs

Cq Uniformity

Users can expect minimal well-to-well variability in Array Tape, providing The IntelliQube demonstrated a total standard deviation (SD) of 0.087 cycles when 768 replicate wells each containing 2048 copies of a NIST calibrated human – gDNA reference standard were analyzed for a non- transcribed locus in hgDNA.

Figure 1. Cq Uniformity <0.09 SD

Dynamic Range

The IntelliQube can support assays across a large range of input concentrations while maintaining data integrity. When analyzing a 1000 bp synthetic target serially diluted across eight orders of magnitude, the IntelliQube demonstrated a linear dynamic range of quantifications between 4x107 to 40 copies/reaction.

Figure 2. Linear dynamic range of quantification from 4x10⁷ to 40 copies/reaction

Resolution

Minimal differences in target quality can be accurately resolved between reaction wells on the IntelliQube. Using NIST calibrated hgDNA reference standard, reactions were prepared to represent 1.2 fold differences in template concentration. When analyzed for a non-transcribed locus in hgDNA, the IntelliQube demonstrated the ability to resolve 1.2 fold differences with greater than 97% sensitivity and specificity.

Figure 3. 1.2 fold resolution

Sensitivity

The IntelliQube provides the sensitivity necessary to amplify and detect even 1-2 copies of target DNA in a reaction well. Although the theoretical limit of detection (LOD) provided by Poisson statistics is limited to three molecules, IntelliQube has demonstrated the ability to detect as little as 1-2 molecules of hgDNA per reaction.

IntelliQube Performance Specifications

Dimensions (W x D x H)	49" x 34" x 75" (with cart)
Weight	855 pounds
Input Voltage	120/240 VAC, 15/7.5 A
Frequency	50-60 Hz
Operating Temperature	15-30°C
Operating Humidity	20-80% Relative Humidity, non-condensing
Air Input	80-100 psi, bursts up to 15 SCFM
Water Input	Reverse Osmosis or Deionized
Certifications	Conforms to the European Machinery Directive (2006/42/EC) and the EMC Directive (2004/108/EC). For Research Use Only. Not for use in Diagnostic Procedures.
Estimated End-point PCR Throughput Per 8 Hours (With Hydrocycler2)	Sixty Five 384-well arrays 24,960 data points

Temperature uniformity

Specification	Peltier block type
Block configuration	384/768-well Array Tape
Temperature range	20 - 100°C
Temperature accuracy	±0.25°C
Temperature uniformity	±0.5°C at 60°C
Heating ramp rate	3.0°C/sec
Cooling rate	3.0°C/sec

Detection

LEDs (460-645 nm)
Dye channel	1	2	3	4	5
Excitation filter (nm)	480	530	548	580	620
Emission Filter (nm)	510	565	580	625	705
Dyes with expected compatability	FAM™	JOE™ VIC^®/HEX^™ Cal Fluor^® Orange 560	NED™ TAMRA^™ Cal Fluor^® Red 590	ROX™ Cal Fluor^® Red 610 Texas Red^®	Cy5^® Quasar^® 670