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BHQplus™ Probes


The BHQplus™ probe is a novel compact probe for qPCR, modified with a duplex stabilizing technology and terminated with Biosearch’s Black Hole Quencher® dye. What differentiates the BHQplus probe from other traditional probes is that it permits the design of shorter oligonucleotides, typically 15 - 25 bases in length, for detecting more difficult target sequences. The enhanced specificity of BHQplus probes make them perfect for genotyping single nucleotide polymorphisms.

BHQplus probes feature:
  Scatterplot
  Scatterplot of a BHQplus SNP Assay, resolving the respective genotypes
 

Looking for more information? View our poster Genotyping with BHQplus probes for additional details.

  • Small but powerful: duplex stabilizers permit the design of shorter dual-labeled probes
  • High fidelity: shorter sequences offer an increased barrier against hybridizing to a mismatch
  • Proven performance: the Black Hole Quencher dye extinguishes fluorescence until target hybridization
  • A mouse-click away: design your BHQplus probe through Biosearch’s free, web-based RealTimeDesign™ software
  • Bottom line: An economical alternative for probe-based SNP Genotyping
Please be aware that sequences longer than 25 bases may have been designed as a traditional probe and are unlikely to work well in BHQplus form. Instead, consider ordering a BHQ® probe instead if such a sequence already has a predicted melting temperature of 70 °C. Conversely, sequences shorter than 15 bases may have been designed as a Minor Groove Binder (MGB™) probe and should first be analyzed for compatibility with the BHQplus chemistry. Please contact techsupport@biosearchtech.com with any questions regarding probe design.

NOTES:
Building Probes with Wobble/Degenerate Bases
(What are Wobbles?)
To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc.

See also:
qPCR Assays | SNP AssaysDual-labeled BHQ® Probes | Molecular Beacons | Scorpions® Primers | Dye Calibration Standards | Dyes from Biosearch Technologies

Biosearch Technologies offers RealTimeDesign software, a FREE assay design program hosted on the web.  Start designing your BHQplus probes and primer pairs today! Access RealTimeDesign



Product Listings

Catalog # Item Name Price Size/Scale Note
DLO-FBP-5 5’ FAM BHQplus™ Probe $195.00 50 nmol Provides 10 nmol pure.
DLO-FBP-2 5’ FAM BHQplus™ Probe $350.00 200 nmol Provides 20 nmol pure.
DLO-FBP-1 5’ FAM BHQplus™ Probe $600.00 1 µmol Provides 60 nmol pure.
DLO-TBP-5 5’ TET BHQplus™ Probe $195.00 50 nmol Provides 10 nmol pure.
DLO-TBP-2 5’ TET BHQplus™ Probe $350.00 200 nmol Provides 20 nmol pure.
DLO-TBP-1 5’ TET BHQplus™ Probe $600.00 1 µmol Provides 60 nmol pure.
DLO-GBP-5 5’ CAL Fluor Gold 540 BHQplus™ Probe $195.00 50 nmol Provides 10 nmol pure.
DLO-GBP-2 5’ CAL Fluor Gold 540 BHQplus™ Probe $350.00 200 nmol Provides 20 nmol pure.
DLO-GBP-1 5’ CAL Fluor Gold 540 BHQplus™ Probe $600.00 1 µmol Provides 60 nmol pure.
DLO-CBP-5 5’ CAL Fluor Orange 560 BHQplus™ Probe $195.00 50 nmol Provides 10 nmol pure.
DLO-CBP-2 5’ CAL Fluor Orange 560 BHQplus™ Probe $350.00 200 nmol Provides 20 nmol pure.
DLO-CBP-1 5’ CAL Fluor Orange 560 BHQplus™ Probe $600.00 1 µmol Provides 60 nmol pure.
DLO-RBP-5 5’ CAL Fluor Red 610 BHQplus™ Probe $195.00 50 nmol Provides 10 nmol pure.
DLO-RBP-2 5’ CAL Fluor Red 610 BHQplus™ Probe $350.00 200 nmol Provides 20 nmol pure.
DLO-RBP-1 5’ CAL Fluor Red 610 BHQplus™ Probe $600.00 1 µmol Provides 60 nmol pure.
DLO-QBP-5 5’ Quasar 670 BHQplus™ Probe $195.00 50 nmol Provides 10 nmol pure.
DLO-QBP-2 5’ Quasar 670 BHQplus™ Probe $350.00 200 nmol Provides 20 nmol pure.
DLO-QBP-1 5’ Quasar 670 BHQplus™ Probe $600.00 1 µmol Provides 60 nmol pure.
P2-5 Primer Pair, (with probe purchase) Salt-Free (up to 30 bases each) $14.00 50 nmol Provides 20-40 nmol.
P2-2 Primer Pair, (with probe purchase) Salt-Free (up to 30 bases each) $25.00 200 nmol Provides 80-160 nmol.
P2-1 Primer Pair, (with probe purchase) Salt-Free (up to 30 bases each) $40.00 1 µmol Provides 400-800 nmol.
P2C-5 Primer Pair, (with probe purchase) Cartridge Purified (up to 30 bases each) $30.00 50 nmol Provides 11.25-22.5 nmol pure.
P2C-2 Primer Pair, (with probe purchase) Cartridge Purified (up to 30 bases each) $35.00 200 nmol Provides 45-90 nmol pure.
P2C-1 Primer Pair, (with probe purchase) Cartridge Purified (up to 30 bases each) $50.00 1 µmol Provides 225-450 nmol pure.



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NOTE: The pricing shown is for primer pairs ordered with probes only.
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