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ValuMix Assay for SNP Genotyping


ValuMix AssayBiosearch Technologies is now offering ValuMix™ Assays for genotyping Single Nucleotide Polymorphisms (SNP).  This new product offering comprises custom BHQplus® probes and primers combined within a single tube. This formulation minimizes pipetting error and simplifies your protocol by providing mixed oligonucleotides ready for SNP genotyping.

SNP assays comprise two BHQplus probes and primers formulated together as a set. BHQplus probes contain modified pyrimidine residues, a duplex stabilizing chemistry, that permit the design of shorter oligonucleotides, typically 15 - 25 bases in length. Shorter probes are more discriminating and so the enhanced specificity of BHQplus probes make them perfect for SNP genotyping.

Please refer to the Product Listings tab to view all dye-quencher combinations and delivery quantities available for custom BHQplus SNP assays.

Not familiar with probe-based SNP genotyping? Here are some resources:


NOTES:
BHQplus Probe Format Compatibility

Sequences that may have been designed as a Minor Groove Binder (MGB™) probe should first be analyzed for compatibility with the BHQplus chemistry, particularly if they are shorter than 15 bases in length. Please contact techsupport@biosearchtech.com with any questions regarding probe design.

Building Probes with Degenerate Bases (What are Wobbles?)
To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses or brackets, e.g. R or V instead of (A/G) or [A/G/C], etc.


See also:
BHQ® Probes | BHQplus® Probes | qPCR Assays | Molecular Beacons | Scorpions® Primers | Dye Calibration Standards | Dyes from Biosearch Technologies




Product Listings

Catalog # Item Name Price Note
FTBP-5 FAM/TET BHQplus SNP Probe Pair w/ Primers, Mixed $395.00 5 nmol probe, 22.5 nmol primer
FTBP-2 FAM/TET BHQplus SNP Probe Pair w/ Primers, Mixed $270.00 2 nmol probe, 9 nmol primer
FTBP-12 FAM/TET BHQplus SNP Probe Pair w/ Primers, Mixed $529.00 12 nmol probe, 54 nmol primer
FGBP-5 FAM/CAL Fluor Gold 540 BHQplus SNP Probe Pair w/ Primers, Mixed $395.00 5 nmol probe, 22.5 nmol primer
FGBP-2 FAM/CAL Fluor Gold 540 BHQplus SNP Probe Pair w/ Primers, Mixed $270.00 2 nmol probe, 9 nmol primer
FGBP-12 FAM/CAL Fluor Gold 540 BHQplus SNP Probe Pair w/ Primers, Mixed $529.00 12 nmol probe, 54 nmol primer
FCBP-5 FAM/CAL Fluor Orange 560 BHQplus SNP Probe Pair w/ Primers, Mixed $395.00 5 nmol probe, 22.5 nmol primer
FCBP-2 FAM/CAL Fluor Orange 560 BHQplus SNP Probe Pair w/ Primers, Mixed $270.00 2 nmol probe, 9 nmol primer
FCBP-12 FAM/CAL Fluor Orange 560 BHQplus SNP Probe Pair w/ Primers, Mixed $529.00 12 nmol probe, 54 nmol primer

Quick Order

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Probe 1
FAM
BHQ-1 plus
All probes are RP HPLC purified

Probe 2
All probes are RP HPLC purified
Forward Primer
All primers are Salt-Free
Reverse Primer
All primers are Salt-Free

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