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Product Info

The BHQplus® probe is a novel compact probe for qPCR, modified with a duplex stabilizing technology and terminated with Biosearch’s Black Hole Quencher® dye. What differentiates the BHQplus probe from other traditional probes is that it permits the design of shorter oligonucleotides, typically 15 - 25 bases in length, for detecting more difficult target sequences. The enhanced specificity of BHQplus probes make them perfect for genotyping single nucleotide polymorphisms.

BHQplus probes are offered in two convenient deliveries. For BHQplus probes and primers delivered in individual tubes select just “BHQplus probes”, however if you would like the probes and primers blended into a single tube select “ValuMix Assay for SNP genotyping”.

ValuMix™ Assays for SNP genotyping comprises two custom BHQplus® probes and two primers combined within a single tube. This formulation minimizes pipetting error and simplifies your protocol by providing mixed oligonucleotides ready for SNP genotyping.

Please refer to the Product Listings tab to view all dye-quencher combinations and delivery quantities available.

BHQplus probes feature:

  Scatterplot
  Scatterplot of a BHQplus SNP Assay, resolving the respective genotypes
 

Looking for more information? View our poster Genotyping with BHQplus® probes for additional details.

  • Small but powerful: duplex stabilizers permit the design of shorter dual-labeled probes
  • High fidelity: shorter sequences offer an increased barrier against hybridizing to a mismatch
  • Proven performance: the Black Hole Quencher dye extinguishes fluorescence until target hybridization
  • A mouse-click away: design your BHQplus probe through Biosearch’s free, web-based RealTimeDesign™ software
  • Bottom line: An economical alternative for probe-based SNP Genotyping

Please be aware that sequences longer than 25 bases may have been designed as a traditional probe and are unlikely to work well in BHQplus form. Instead, consider ordering a BHQ® probe instead if such a sequence already has a predicted melting temperature of 70 °C. Conversely, sequences shorter than 15 bases may have been designed as a Minor Groove Binder (MGB™) probe and should first be analyzed for compatibility with the BHQplus chemistry. Please contact techsupport@biosearchtech.com with any questions regarding probe design.

NOTES:
Building Probes with Wobble/Degenerate Bases
(What are Wobbles?)
To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc.

Biosearch Technologies offers RealTimeDesign™ software, a FREE assay design program hosted on the web. Start designing your BHQplus probes and primer pairs today! Access RealTimeDesign

Product Listing

Catalog # Item Name Price Note
{{listedItem.CatalogId}} {{listedItem.CatalogId}}
{{listedItem.Price|currency}} TBD

Oligo set {{$index + 1}}

{{error.message}}
You need to change the AssayName because it has already been used.
Field required.
ValuProbe
Field required.
Field required.
This sequence is too short. Click here for help.
This probe sequence is shorter than our recommended criteria for probe length. You may want to consider ordering this sequence as a BHQplus® probe BHQplus® probe instead.
You may want to consider ordering an internally quenched probe for sequences 30 bases or longer.
A surcharge has been applied to the price of this probe because the sequence length is longer than 35 bases.
A surcharge has been applied to the price of this probe because the sequence length is longer than 50 bases.
This oligo sequence is too long. Please contact customer service info@biosearchtech.com for a quote or click here for help.
This probe sequence is too long. Please contact customer service info@biosearchtech.com for a quote or click here for help.
Are you trying to order a Molecular Beacon? Click here to continue Click here to continue
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
The internal modification you used in the sequence is not available with this 5' Modification. You need to remove it from your sequence.
Are you trying to include RNA bases? RNA bases are not allowed in standard BHQ probes and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in standard BHQplus probes and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
RNA bases are not allowed in standard Molecular Beacons. Please use the custom oligo form custom oligo form to order these modifications or click here for help.
Inosine modifications are not allowed in standard BHQ probes. Please order on the custom oligo form custom oligo form or click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
Illegal character(s) were entered. Please review your sequence information.
Internal modifications are not allowed in standard Molecular Beacons. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
Internal modifications are not allowed in standard BHQplus probes. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
The sequence contains open brackets. Please, verify your sequence.
Field required.
add
Probe Scale
Field required.

Purification
Item Price TBD
Field required.
This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Inosine modifications must be included in brackets such as [I] or [i].
Internal modifications are not allowed in standard primers. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
No special modifications are allowed in BHQplus® probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
Illegal character(s) were entered. Please review your sequence information.
Item Price
Field required.
This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Inosine modifications must be included in brackets such as [I] or [i].
Internal modifications are not allowed in standard primers. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
No special modifications are allowed in BHQplus® probes and primers. Use the custom oligo order form custom oligo order form to order sequences with specialty modifications or click here for help.
Illegal character(s) were entered. Please review your sequence information.
Item Price
Primer Scale
Field required.
Clear Scale

Field required.
You Are Ordering:
Probe Catalog #: {{order.probe1.fivePrime.threePrime.item.catalogId}} Primer Catalog #: {{order.primer.item.catalogId}} Assay Subtotal: {{(order.probe1.fivePrime.threePrime.item.price + order.primer.primer2.price + order.primer.primer1.price)*order.quantity | currency}} QTY ANOTHER Assay
Max Assay forms reached, Please add current assays to your cart, then return to form to continue adding more assays
Note

Subtotal: {{ctrl.getTotalPrice() | currency}} add to cart add to cart

Assay #{{$index + 1}}

Field required.
You need to change the AssayName because it has already been used.
Probe 1 Name
5’ Modification
Field required.
This sequence is too short. Click here for help.
This probe sequence is longer than our recommended criteria for probe length. You may want to consider ordering this sequence as a Dual-labeled BHQ® probe Dual-labeled BHQ® probe instead.
This probe sequence exceeds the 30 base limit for ValuMix assays for SNP genotyping. Please order this oligo as a BHQplus® probe BHQplus® probe or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Probes and primers for this type of ValuMix Assay cannot include internal modifications. Click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in ValuMix assays and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide custom oligonucleotide or click here for help.
The sequence contains open brackets. Please, verify your sequence.
3’ Modification
Purification
Probe 2 Name
Field required.
Field required.
This sequence is too short. Click here for help.
This probe sequence is longer than our recommended criteria for probe length. You may want to consider ordering this sequence as a Dual-labeled BHQ® probe Dual-labeled BHQ® probe instead.
This probe sequence exceeds the 30 base limit for ValuMix assays for SNP genotyping. Please order this oligo as a BHQplus® probe BHQplus® probe or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
The internal modification you used in the sequence is not available with this 5' Modification. You need to remove it from your sequence.
Probes and primers for this type of ValuMix Assay cannot include internal modifications. Click here for help.
Are you trying to include RNA bases? RNA bases are not allowed in ValuMix assays and so the R character will be treated as a DNA wobble instead (A/G). Please use the custom oligo form custom oligo form to order these modifications or click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide or click here for help.
The sequence contains open brackets. Please, verify your sequence.
3’ Modification
Probe Scale
Field required.

Purification
Item Price TBD

Forward Primer

Sequence Name
Field required.
This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Probes and primers for this type of ValuMix Assay cannot include internal modifications. Click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide custom oligonucleotide or click here for help.
Purification All primers are Salt-Free

Reverse Primer

Sequence Name
Field required.
This sequence is too short. Click here for help.
This primer sequence is too long. Use the custom oligo order form custom oligo order form to order a long unlabeled oligo or click here for help.
This oligo contains a number of 'wobbles'. Please consider limiting this to one trinucleotide wobble and one dinucleotide wobble in two different locations or three dinucleotide wobbles in three different locations such that there is a maximum of 6 variants in a single oligonucleotide.
Probes and primers for this type of ValuMix Assay cannot include internal modifications. Click here for help.
An invalid character has been entered into this sequence. Please order this sequence as a custom oligonucleotide custom oligonucleotide custom oligonucleotide or click here for help.
Purification All primers are Salt-Free
{{error.message}}
You Are Ordering:
Probe Catalog #: {{order.probe2.fivePrime.threePrime.item.catalogId}} Assay Subtotal: {{(order.probe2.fivePrime.threePrime.item.price)*order.quantity | currency}} QTY ANOTHER Assay
Max Assay forms reached, Please add current assays to your cart, then return to form to continue adding more assays
Note

Subtotal: {{ctrl.getTotalPrice() | currency}} add to cart add to cart