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Stellaris® FISH Probes, Human GAPDH_intron with CAL Fluor® Red 590 Dye consists of a set of dye-labeled oligos mixed and pooled into a final delivered amount of 5 nmol, which yields approximately 200-400 hybridizations under standard conditions. Designed to detect GAPDH transcripts in specimens using fluorescence in situ hybridization (FISH). Design Criteria: Product was designed against glyceraldehyde-3-phosphate dehydrogenase GAPDH a.k.a. G3PD; GAPD; HEL-S-162eP (NCBI gene ID:2597) and nucleotides 5080-5319, 5372-5728, 5980-7003, 7104-7193, 7301-7429, 7521-7610, 7727-7818, 7901-8093, 8507-8610 of NG_007073.2. This is an inclusive probe set designed to detect all known GAPDH pre-mRNAs. The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.

Properties:

  • Absorption Maximum (Lambda Max):

    569

  • Fluorescence Maximum:

    591

Product usage:

    Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month. For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month. Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols.

Storage and handling:

  • Shipping conditions:

    Ambient

  • Storage conditions:

    +2 to +8 °C

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